ArticleBMC genomics2012
Decreased miR-192 expression in peripheral blood of asthmatic individuals undergoing an allergen inhalation challenge.
Article in BMC genomics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Trial
- MicroRNA expression in response to controlled exposure to diesel exhaust: attenuation by the antioxidant N-acetylcysteine in a randomized crossover study.Environmental health perspectives · 2013Trial
- MicroRNAs-A Promising Tool for Asthma Diagnosis and Severity Assessment: A Systematic Review.Journal of personalized medicine · 2022Review
- Role of microRNAs in type 2 diseases and allergen-specific immunotherapy.Frontiers in allergy · 2022Review
- Baicalin relievesMolecular medicine reports · 2021Article
- Emerging Role of MiR-192-5p in Human Diseases.Frontiers in pharmacology · 2021Review
- MicroRNAs in Asthma and Respiratory Infections: Identifying Common Pathways.Allergy, asthma & immunology research · 2020Review
- T Follicular Helper Cell Subsets and the Associated Cytokine IL-21 in the Pathogenesis and Therapy of Asthma.Frontiers in immunology · 2019Review
- Posttranscriptional Regulation of Interleukin-33 Expression by MicroRNA-200 in Bronchial Asthma.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Selected microRNA-192 mutant indicates association with several function genes in bovine cells.Genes & genomics · 2018Article
- The role of microRNAs in chronic respiratory disease: recent insights.Biological chemistry · 2018Review
- Towards precision medicine: The application of omics technologies in asthma management.F1000Research · 2018Article
- Precision Medicine in Targeted Therapies for Severe Asthma: Is There Any Place for "Omics" Technology?BioMed research international · 2018Review
- Non-Coding RNAs in Pediatric Airway Diseases.Genes · 2017Review
- MicroRNAs as therapeutics for future drug delivery systems in treatment of lung diseases.Drug delivery and translational research · 2017Review
- Circulating microRNAs as Potential Biomarkers of Infectious Disease.Frontiers in immunology · 2017Review
- Targeting MicroRNA Function in Respiratory Diseases: Mini-Review.Frontiers in physiology · 2016Review
- Differential inflammatory microRNA and cytokine expression in pulmonary sarcoidosis.Archivum immunologiae et therapiae experimentalis · 2015Article
- Usefulness of circulating microRNAs for the prediction of early preeclampsia at first-trimester of pregnancy.Scientific reports · 2014Article
- Th17/Treg ratio derived using DNA methylation analysis is associated with the late phase asthmatic response.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2014Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
backgroundMicroRNAs are small non-coding RNAs that regulate gene expression at the post-transcriptional level. While they have been implicated in various diseases, the profile changes in allergen inhalation challenge are not clarified in human. We aimed to evaluate changes in the microRNA profiles in the peripheral blood of asthmatic subjects undergoing allergen inhalation challenge.
resultsSeven mild asthmatic subjects participated in the allergen inhalation challenge. In addition, four healthy control subjects (HCs) were recruited. MicroRNA profiles in peripheral blood samples (pre-challenge and 2 hours post-challenge) were measured by the NanoString nCounter assay to determine changes in miRNA levels as these asthmatic subjects underwent an allergen inhalation challenge. One common miRNA, miR-192, was significantly expressed in both comparisons; HCs vs. pre-challenge and pre- vs. post-challenge, showing that miR-192 was significantly under-expressed in asthmatics compared to HCs and decreased in post-challenge at an FDR of 1%. Cell-specific statistical deconvolution attributed miR-192 expression in whole blood to PBMCs. MiR-192 was technically validated using real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR) showing that the level in asthmatics (pre-challenge) was significantly lower than HCs and that post-challenge was significantly lower than pre-challenge. The normalized relative miR-192 expression quantified using RT-qPCR specific to PBMCs was also validated. Ontology enrichment and canonical pathway analyses for target genes suggested several functions and pathways involved in immune response and cell cycle.
conclusionsThe miRNA profile in peripheral blood was altered after allergen inhalation challenge. Change in miR-192 levels may be implicated in asthma mechanisms. These results suggest that allergen inhalation challenge is a suitable method to characterize peripheral miRNA profiles and potentially elucidate the mechanism of human asthma.
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