ArticleProteome science2012
An individual urinary proteome analysis in normal human beings to define the minimal sample number to represent the normal urinary proteome.
Article in Proteome science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Urinary Collectrin (TMEM27) as Novel Marker for Acute Kidney Injury.Life (Basel, Switzerland) · 2022Article
- HBFP: a new repository for human body fluid proteome.Database : the journal of biological databases and curation · 2021Article
- Human body-fluid proteome: quantitative profiling and computational prediction.Briefings in bioinformatics · 2021Review
- Comprehensive Analysis of Individual Variation in the Urinary Proteome Revealed Significant Gender Differences.Molecular & cellular proteomics : MCP · 2019Article
- Article
- Effect of Sweetened Dried Cranberry Consumption on Urinary Proteome and Fecal Microbiome in Healthy Human Subjects.Omics : a journal of integrative biology · 2018Article
- More complete polarization of renal tubular epithelial cells by artificial urine.Cell death discovery · 2018Article
- Urinary neprilysin in the critically ill patient.BMC nephrology · 2017Article
- Article
- Bladder cancer cells secrete while normal bladder cells express but do not secrete AGR2.Oncotarget · 2016Article
- Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma.Clinical cancer research : an official journal of the American Association for Cancer Research · 2015Article
- Article
- A Proteomic Analysis of Individual and Gender Variations in Normal Human Urine and Cerebrospinal Fluid Using iTRAQ Quantification.PloS one · 2015Article
- A standardized and reproducible urine preparation protocol for cancer biomarkers discovery.Biomarkers in cancer · 2014Article
- Urinary Apolipoprotein C3 Is a Potential Biomarker for Alzheimer's Disease.Dementia and geriatric cognitive disorders extraArticle
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelled
backgroundThe urinary proteome has been widely used for biomarker discovery. A urinary proteome database from normal humans can provide a background for discovery proteomics and candidate proteins/peptides for targeted proteomics. Therefore, it is necessary to define the minimum number of individuals required for sampling to represent the normal urinary proteome.
methodsIn this study, inter-individual and inter-gender variations of urinary proteome were taken into consideration to achieve a representative database. An individual analysis was performed on overnight urine samples from 20 normal volunteers (10 males and 10 females) by 1DLC/MS/MS. To obtain a representative result of each sample, a replicate 1DLCMS/MS analysis was performed. The minimal sample number was estimated by statistical analysis.
resultsFor qualitative analysis, less than 5% of new proteins/peptides were identified in a male/female normal group by adding a new sample when the sample number exceeded nine. In addition, in a normal group, the percentage of newly identified proteins/peptides was less than 5% upon adding a new sample when the sample number reached 10. Furthermore, a statistical analysis indicated that urinary proteomes from normal males and females showed different patterns. For quantitative analysis, the variation of protein abundance was defined by spectrum count and western blotting methods. And then the minimal sample number for quantitative proteomic analysis was identified.
conclusionsFor qualitative analysis, when considering the inter-individual and inter-gender variations, the minimum sample number is 10 and requires a balanced number of males and females in order to obtain a representative normal human urinary proteome. For quantitative analysis, the minimal sample number is much greater than that for qualitative analysis and depends on the experimental methods used for quantification.
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