Evidence map›Paper›PMID 23170185›Full record

ArticleViruses2012

Construction of a high titer infectious HIV-1 subtype C proviral clone from South Africa.

Graeme B Jacobs, Stefanie Bock, Anita Schuch, Rebecca Moschall, Eva-Maria Schrom, Juliane Zahn, Christian Reuter, Wolfgang Preiser, Axel Rethwilm, Susan Engelbrecht and 2 more

Open access · goldAbstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 85% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 2 countries.

Graeme B JacobsInstitut für Virologie und Immunbiologie, Universität Würzburg, Versbacher Straße 7, 97078 Würzburg, Germany. graeme@sun.ac.za
Stefanie Bock
Anita Schuch
Rebecca Moschall
Eva-Maria Schrom
Juliane Zahn
Christian Reuter
Wolfgang Preiser
Axel Rethwilm
Susan Engelbrecht
Thomas Kerkau
Jochen Bodem
University of Würzburg · DENational Health Laboratory Service · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Human Immunodeficiency Virus type 1 (HIV-1) subtype C is currently the predominant subtype worldwide. Cell culture studies of Sub-Saharan African subtype C proviral plasmids are hampered by the low replication capacity of the resulting viruses, although viral loads in subtype C infected patients are as high as those from patients with subtype B. Here, we describe the sequencing and construction of a new HIV-1 subtype C proviral clone (pZAC), replicating more than one order of magnitude better than the previous subtype C plasmids. We identify the env-region for being the determinant for the higher viral titers and the pZAC Env to be M-tropic. This higher replication capacity does not lead to a higher cytotoxicity compared to previously described subtype C viruses. In addition, the pZAC Vpu is also shown to be able to down-regulate CD4, but fails to fully counteract CD317.

Indexed as

Aged, 80 and overCloning, MolecularDNA, ViralHIV-1HIV InfectionsHumansMaleMolecular Sequence DataProvirusesSequence Analysis, DNASouth AfricaViral TropismVirus ReplicationDNA, ViralCD317CD4HIV-1proviral plasmidresistance assayssubtype Cviral replicationVpu

Identifiers

PMID23170185
PMCPMC3499832
OpenAlexW2120655648

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.