ArticlemBio2012
Unexpected diversity of signal peptides in prokaryotes.
Article in mBio, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- TlyC, a conserved hemolysin inMicrobiology spectrum · 2025Article
- Signal Peptides: From Molecular Mechanisms to Applications in Protein and Vaccine Engineering.Biomolecules · 2025Review
- Heterologous protein exposure and secretion optimization in Mycoplasma pneumoniae.Microbial cell factories · 2024Article
- Developing a platform for secretion of biomolecules in Mycoplasma feriruminatoris.Microbial cell factories · 2024Article
- Pathogenicity and virulence ofVirulence · 2022Review
- Construction and Application of a Plasmid-Based Signal Peptide Library for Improved Secretion of Recombinant Proteins withMicroorganisms · 2022Article
- Article
- Insights into phosphatase-activated chemical defense in a marine sponge holobiont.RSC chemical biology · 2021Article
- Article
- Direct detection of intactClinical mass spectrometry (Del Mar, Calif.) · 2020Article
- Detection, characterization and evolutionary aspects of S54LP of SP (SAP54 Like Protein of Sesame Phyllody): a phytoplasma effector molecule associated with phyllody development in sesame (Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Evolutionary Interplay between Symbiotic Relationships and Patterns of Signal Peptide Gain and Loss.Genome biology and evolution · 2018Article
- Tracking Proteins Secreted by Bacteria: What's in the Toolbox?Frontiers in cellular and infection microbiology · 2017Review
- Secretome of obligate intracellular Rickettsia.FEMS microbiology reviews · 2015Review
- Structure-function of cyanobacterial outer-membrane protein, Slr1270: homolog of Escherichia coli drug export/colicin import protein, TolC.FEBS letters · 2014Article
- The use of signal peptide domains as vaccine candidates.Human vaccines & immunotherapeutics · 2014Review
- QARIP: a web server for quantitative proteomic analysis of regulated intramembrane proteolysis.Nucleic acids research · 2013Article
- How many signal peptides are there in bacteria?Environmental microbiology · 2013Article
- Arming the troops: Post-translational modification of extracellular bacterial proteins.Science progressReview
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelledSignal peptides are a cornerstone mechanism for cellular protein localization, yet until now experimental determination of signal peptides has come from only a narrow taxonomic sampling. As a result, the dominant view is that Sec-cleaved signal peptides in prokaryotes are defined by a canonical AxA motif. Although other residues are permitted in the motif, alanine is by far the most common. Here we broadly examine proteomics data to reveal the signal peptide sequences for 32 bacterial and archaeal organisms from nine phyla and demonstrate that this alanine preference is not universal. Discoveries include fundamentally distinct signal peptide motifs from Alphaproteobacteria, Spirochaetes, Thermotogae and Euryarchaeota. In these novel motifs, alanine is no longer the dominant residue but has been replaced in a different way for each taxon. Surprisingly, divergent motifs correlate with a proteome-wide reduction in alanine. Computational analyses of ~1,500 genomes reveal numerous major evolutionary clades which have replaced the canonical signal peptide sequence with novel motifs. IMPORTANCE: This article replaces a widely held general model with a more detailed model describing phylogenetically correlated variation in motifs for Sec secretion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.