ArticleJournal of biochemical and molecular toxicology2013
Optimization of fluorescence assay of cellular manganese status for high throughput screening.
Article in Journal of biochemical and molecular toxicology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 9 citations in OpenAlex.
- A Cell-Based Protocol to Assess Manganese Content and Relative Transport Activity of Manganese Transporters.Bio-protocol · 2026Article
- Prolonged duration induces divergent transcriptomic responses to manganese, distinct from concentration effects, in an SH-SY5Y neurotoxicity model.Neurotoxicology · 2026Article
- Identification of Three Small Molecules That Can Selectively Influence Cellular Manganese Levels in a Mouse Striatal Cell Model.Molecules (Basel, Switzerland) · 2021Article
- Identification of a selective manganese ionophore that enables nonlethal quantification of cellular manganese.The Journal of biological chemistry · 2020Article
- Manganese-Induced Parkinsonism and Parkinson's Disease: Shared and Distinguishable Features.International journal of environmental research and public health · 2015Review
- A novel manganese-dependent ATM-p53 signaling pathway is selectively impaired in patient-based neuroprogenitor and murine striatal models of Huntington's disease.Human molecular genetics · 2015Article
- Untargeted metabolic profiling identifies interactions between Huntington's disease and neuronal manganese status.Metallomics : integrated biometal science · 2015Article
- Cellular manganese content is developmentally regulated in human dopaminergic neurons.Scientific reports · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The advent of high throughput screening (HTS) technology permits identification of compounds that influence various cellular phenotypes. However, screening for small molecule chemical modifiers of neurotoxicants has been limited by the scalability of existing phenotyping assays. Furthermore, the adaptation of existing cellular assays to HTS format requires substantial modification of experimental parameters and analysis methodology to meet the necessary statistical requirements. Here we describe the successful optimization of the Cellular Fura-2 Manganese Extraction Assay (CFMEA) for HTS. By optimizing cellular density, manganese (Mn) exposure conditions, and extraction parameters, the sensitivity and dynamic range of the fura-2 Mn response was enhanced to permit detection of positive and negative modulators of cellular manganese status. Finally, we quantify and report strategies to control sources of intra- and interplate variability by batch level and plate-geometric level analysis. Our goal is to enable HTS with the CFMEA to identify novel modulators of Mn transport.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.