ArticleProceedings of the National Academy of Sciences of the United States of America2012
Chemogenomic approach identified yeast YLR143W as diphthamide synthetase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- GWAS in a Collection of Bulgarian Old and Modern Bread Wheat Accessions Uncovers Novel Genomic Loci for Grain Protein Content and Thousand Kernel Weight.Plants (Basel, Switzerland) · 2024Article
- Translational fidelity and growth of Arabidopsis require stress-sensitive diphthamide biosynthesis.Nature communications · 2022Article
- Quantitative proteomics reveals protein dysregulation during T cell activation in multiple sclerosis patients compared to healthy controls.Clinical proteomics · 2022Article
- Open Issues for Protein Function Assignment inGenes · 2021Article
- Iron in Translation: From the Beginning to the End.Microorganisms · 2021Review
- Identification of the transcription factor Miz1 as an essential regulator of diphthamide biosynthesis using a CRISPR-mediated genome-wide screen.PLoS genetics · 2020Article
- The asymmetric function of Dph1-Dph2 heterodimer in diphthamide biosynthesis.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2019Article
- Complex Evolutionary History of Translation Elongation Factor 2 and Diphthamide Biosynthesis in Archaea and Parabasalids.Genome biology and evolution · 2018Article
- Article
- Importance of diphthamide modified EF2 for translational accuracy and competitive cell growth in yeast.PloS one · 2018Article
- Evolution of the archaeal and mammalian information processing systems: towards an archaeal model for human disease.Cellular and molecular life sciences : CMLS · 2017Review
- Cbr1 is a Dph3 reductase required for the tRNA wobble uridine modification.Nature chemical biology · 2016Article
- A diphtheria toxin resistance marker for in vitro and in vivo selection of stably transduced human cells.Scientific reports · 2015Article
- Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis.Journal of the American Chemical Society · 2014Article
- Dph3 is an electron donor for Dph1-Dph2 in the first step of eukaryotic diphthamide biosynthesis.Journal of the American Chemical Society · 2014Article
- Article
- The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network.PLoS genetics · 2013Article
- The biosynthesis and biological function of diphthamide.Critical reviews in biochemistry and molecular biologyReview
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Many genes are of unknown functions in any sequenced genome. A combination of chemical and genetic perturbations has been used to investigate gene functions. Here we present a case that such "chemogenomics" information can be effectively used to identify missing genes in a defined biological pathway. In particular, we identified the previously unknown enzyme diphthamide synthetase for the last step of diphthamide biosynthesis. We found that yeast protein YLR143W is the diphthamide synthetase catalyzing the last amidation step using ammonium and ATP. Diphthamide synthetase is evolutionarily conserved in eukaryotes. The previously uncharacterized human gene ATPBD4 is the ortholog of yeast YLR143W and fully rescues the deletion of YLR143W in yeast.
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