ArticleMolecular ecology resources2013
The effect of plant identity and the level of plant decay on molecular gut content analysis in a herbivorous soil insect.
Article in Molecular ecology resources, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed.
- Article
- Article
- What's on the menu? A novel molecular gut content analysis to investigate the feeding behavior of phytophagous insects.Ecology and evolution · 2024Article
- Puke or poop? Comparison of regurgitate and faecal samples to infer alpine grasshopper (Ecology and evolution · 2023Article
- Laboratory protocol is important to improve the correlation between target copies and metabarcoding read numbers of seed DNA in ground beetle regurgitates.Scientific reports · 2023Article
- RNA allows identifying the consumption of carrion prey.Molecular ecology resources · 2022Article
- Article
- Predation onInsects · 2021Article
- A powerful long metabarcoding method for the determination of complex diets from faecal analysis of the European pond turtle (Emys orbicularis, L. 1758).Molecular ecology resources · 2021Article
- Fish as predators and prey: DNA-based assessment of their role in food webs.Journal of fish biology · 2021Review
- Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.PloS one · 2021Article
- Article
- Article
- Host plant use of a polyphagous mirid,Ecology and evolution · 2019Article
- The effect of plant identity and mixed feeding on the detection of seed DNA in regurgitates of carabid beetles.Ecology and evolution · 2018Article
- New universal ITS2 primers for high-resolution herbivory analyses using DNA metabarcoding in both tropical and temperate zones.Scientific reports · 2018Article
- Article
- Evaluation of an automated protocol for efficient and reliable DNA extraction of dietary samples.Ecology and evolution · 2017Article
- The specific host plant DNA detection suggests a potential migration of Apolygus lucorum from cotton to mungbean fields.PloS one · 2017Article
- DNA barcode for the identification of the sand fly Lutzomyia longipalpis plant feeding preferences in a tropical urban environment.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant roots represent an important food source for soil-dwelling animals, but tracking herbivore food choices below-ground is difficult. Here, we present an optimized PCR assay for the detection of plant DNA in the guts of invertebrates, using general plant primers targeting the trnT-F chloroplast DNA region. Based on this assay, we assessed the influence of plant identity on the detectability of ingested plant DNA in Agriotes click beetle larvae. Six different plant species were fed to the insects, comprising a grass, a legume and four nonlegume forbs. Moreover, we examined whether it is possible to amplify DNA of decaying plants and if DNA of decayed plant food is detectable in the guts of the larvae. DNA of the ingested roots could be detected in the guts of the larvae for up to 72-h post-feeding, the maximum digestion time tested. When fed with living plants, DNA detection rates differed significantly between the plant species. This may be ascribed to differences in the amount of plant tissue consumed, root palatability, root morphology and/or secondary plant components. These findings indicate that plant identity can affect post-feeding DNA detection success, which needs to be considered for the interpretation of molecularly derived feeding rates on plants. Amplification of plant DNA from decaying plants was possible as long as any tissue could be retrieved from the soil. The consumption of decaying plant tissue could also be verified by our assay, but the insects seemed to prefer fresh roots over decaying plant material.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.