ArticleInternational journal of ophthalmology2012
Effects of preservation time on proliferative potential of human limbal stem/progenitor cells.
Article in International journal of ophthalmology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- A Simple Method to Dissect, Orientate, and Visualize the Murine Limbal Stem Cell Niche with Cornea and Conjunctiva Attached.Methods in molecular biology (Clifton, N.J.) · 2025Article
- MLN4924 Promotes Self-Renewal of Limbal Stem Cells and Ocular Surface Restoration.Journal of personalized medicine · 2023Article
- Article
- Limbal epithelial stem cell sheets from young donors have better regenerative potential.Scientific reports · 2022Article
- Investigation of factors associated with ABCB5-positive limbal stem cell isolation yields from human donors.The ocular surface · 2020Article
- Corneal cell therapy: with iPSCs, it is no more a far-sight.Stem cell research & therapy · 2018Review
- Pterygial body epithelium domination of pterygial proliferation with TCF4 as a potential key factor.International journal of ophthalmology · 2018Article
- Optimal isolation and xeno-free culture conditions for limbal stem cell function.Investigative ophthalmology & visual science · 2014Article
- Kinetics of expansion of human limbal epithelial progenitor cells in primary culture of explants without feeders.PloS one · 2013Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo determine the proliferative potential and the maintenance of stem cell activity in stored human limbal tissues, and correlate this with the preservation time, cell viability and the expression of stem cell markers.
methodsThirty limbal rims were split into 4 parts and stored in corneal preservation medium at 4°C for 0, 1, 4, or 7 days. The limbal stem cell and mitotic markers P63, CK19, proliferating cell nuclear antigen (PCNA), and Ki67 were determined by immunohistochemical staining. The proliferative potential of limbal epithelial cells was assessed by cell viability, the ability of generating stratified epithelium, and colony forming assay.
resultsThe stored tissues maintained limbal stratified structure to 7 days and exhibited comparable expression level of stem cell and mitotic markers. The proportion of viable cells decreased with the prolonged preservation time, while colony forming efficiency decreased from the 1(st) day and disappeared at the 4(th) day. When inoculated on amniotic membrane, the cells preserved for 1 day formed a stratified epithelium, while the cells from 4 days' preservation formed a discontinuous layer.
conclusionThe colony forming efficiency of limbal epithelial stem/progenitor cells decreased rapidly with the increasing preservation time, while the expression level of markers and capacity of forming epithelial monolayer on amniotic membrane decreased gradually. The limbal epithelial stem cells lost their function earlier than the lost expression level of stem cell markers. This may help us to better choose the appropriate preservation grafts for future limbal stem cell transplantation.
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