ArticleScientific reports2012
TMV-Gate vectors: gateway compatible tobacco mosaic virus based expression vectors for functional analysis of proteins.
Article in Scientific reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Tracking of Tobacco Mosaic Virus in Taxonomically Different Plant Fungi.Journal of fungi (Basel, Switzerland) · 2025Article
- Turnip mosaic virus selectively subverts a PR-5 thaumatin-like, plasmodesmal protein to promote viral infection.The New phytologist · 2025Article
- Agroinfiltration-mediated transient assay for rapid evaluation of constructs in pigeonpea.Biotechnology notes (Amsterdam, Netherlands) · 2025Article
- Trans-complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector.Plant biotechnology journal · 2024Article
- Restriction-ligation-independent production of a TVCV infectious clone and a TVCV-based gene expression vector.Heliyon · 2023Article
- Development of Plant-Based Vaccines for Prevention of Avian Influenza and Newcastle Disease in Poultry.Vaccines · 2022Review
- Three methods for inoculation of viral vectors into plants.Frontiers in plant science · 2022Article
- RNA silencing suppressor-influenced performance of a virus vector delivering both guide RNA and Cas9 for CRISPR gene editing.Scientific reports · 2021Article
- Co-expression With Replicating Vector Overcoming Competitive Effects Derived by a Companion Protease Inhibitor in Plants.Frontiers in plant science · 2021Article
- Transient Production of Human β-Glucocerebrosidase With Mannosidic-TypeFrontiers in plant science · 2021Article
- Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Divergent receptor proteins confer responses to different karrikins in two ephemeral weeds.Nature communications · 2020Article
- Virus-based pharmaceutical production in plants: an opportunity to reduce health problems in Africa.Virology journal · 2019Review
- Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus.Plant biotechnology journal · 2019Article
- Production of Bioactive Recombinant Reteplase by Virus-Based Transient Expression System inFrontiers in plant science · 2019Article
- Assessing Carnivorous Plants for the Production of Recombinant Proteins.Frontiers in plant science · 2019Article
- Dynamin-Like Proteins of Endocytosis in Plants Are Coopted by Potyviruses To Enhance Virus Infection.Journal of virology · 2018Article
- Plant Virus Expression Vectors: A Powerhouse for Global Health.Biomedicines · 2017Review
- Transient Expression of Tetrameric Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana.Frontiers in plant science · 2016Article
- Construction of adenovirus vectors encoding the lumican gene by gateway recombinant cloning technology.International journal of ophthalmology · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant viral expression vectors are advantageous for high-throughput functional characterization studies of genes due to their capability for rapid, high-level transient expression of proteins. We have constructed a series of tobacco mosaic virus (TMV) based vectors that are compatible with Gateway technology to enable rapid assembly of expression constructs and exploitation of ORFeome collections. In addition to the potential of producing recombinant protein at grams per kilogram FW of leaf tissue, these vectors facilitate either N- or C-terminal fusions to a broad series of epitope tag(s) and fluorescent proteins. We demonstrate the utility of these vectors in affinity purification, immunodetection and subcellular localisation studies. We also apply the vectors to characterize protein-protein interactions and demonstrate their utility in screening plant pathogen effectors. Given its broad utility in defining protein properties, this vector series will serve as a useful resource to expedite gene characterization efforts.
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