ArticlePLoS neglected tropical diseases2012
A novel procedure for precise quantification of Schistosoma japonicum eggs in bovine feces.
Article in PLoS neglected tropical diseases, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Diagnosis of Schistosoma infection in non-human animal hosts: A systematic review and meta-analysis.PLoS neglected tropical diseases · 2022Pooled it
- Improved serological testing for bovine schistosomiasis in Eastern Africa.Parasites & vectors · 2026Article
- Field Evaluation of a Point-of-Care Circulating Cathodic Antigen (POC-CCA) Test for the Detection ofEthiopian journal of health sciences · 2026Article
- Schistosomiasis in the Philippines: A Comprehensive Review of Epidemiology and Current Control.Tropical medicine and infectious disease · 2025Review
- Epidemiological Study on Humans, Animals and Snails for Schistosomiasis in Two Endemic Municipalities Nearing Elimination in Bohol, the Philippines.Acta parasitologica · 2025Article
- Improvement of the Diagnostic Method for the Detection of Parasite Eggs Using the Formalin Ethyl Acetate Concentration Technique.Acta parasitologica · 2024Article
- The prevailing infection ofParasitology · 2023Article
- Development and assessment of a novel gold immunochromatographic assay for the diagnosis of schistosomiasis japonica.Frontiers in immunology · 2023Article
- Diagnostic performance of a urine-based ELISA assay for the screening of human schistosomiasis japonica: A comparative study.Frontiers in microbiology · 2022Article
- Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases.Clinical microbiology reviews · 2021Article
- Performance of the point-of-care circulating cathodic antigen test in the diagnosis of schistosomiasis japonica in a human cohort from Northern Samar, the Philippines.Infectious diseases of poverty · 2021Article
- High prevalence of Schistosoma japonicum by perfusion in naturally exposed water buffalo in a region of the Philippines endemic for human schistosomiasis.PLoS neglected tropical diseases · 2021Article
- Prevalence ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Improved Detection of Intestinal Helminth Infections with a Formalin Ethyl-Acetate-Based Concentration Technique Compared to a Crude Formalin Concentration Technique.Tropical medicine and infectious disease · 2021Article
- Asian Schistosomiasis: Current Status and Prospects for Control Leading to Elimination.Tropical medicine and infectious disease · 2019Review
- DNA Diagnostics for Schistosomiasis Control.Tropical medicine and infectious disease · 2018Review
- Epidemiology of soil transmitted Helminth infections in the middle-belt of Ghana, Africa.Parasite epidemiology and control · 2018Article
- Real-time PCR diagnosis of Schistosoma japonicum in low transmission areas of China.Infectious diseases of poverty · 2018Article
- Advances in the Diagnosis of Human Schistosomiasis.Clinical microbiology reviews · 2015Review
- High prevalence of Schistosoma japonicum and Fasciola gigantica in bovines from Northern Samar, the Philippines.PLoS neglected tropical diseases · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
17 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Schistosomiasis japonica is a zoonosis with a number of mammalian species acting as reservoir hosts, including water buffaloes which can contribute up to 75% to human transmission in the People's Republic of China. Determining prevalence and intensity of Schistosoma japonicum in mammalian hosts is important for calculating transmission rates and determining environmental contamination. A new procedure, the formalin-ethyl acetate sedimentation-digestion (FEA-SD) technique, for increased visualization of S. japonicum eggs in bovine feces, is described that is an effective technique for identifying and quantifying S. japonicum eggs in fecal samples from naturally infected Chinese water buffaloes and from carabao (water buffalo) in the Philippines. The procedure involves filtration, sedimentation, potassium hydroxide digestion and centrifugation steps prior to microscopy. Bulk debris, including the dense cellulosic material present in bovine feces, often obscures schistosome eggs with the result that prevalence and infection intensity based on direct visualization cannot be made accurately. This technique removes nearly 70% of debris from the fecal samples and renders the remaining debris translucent. It allows improved microscopic visualization of S. japonicum eggs and provides an accurate quantitative method for the estimation of infection in bovines and other ruminant reservoir hosts. We show that the FEA-SD technique could be of considerable value if applied as a surveillance tool for animal reservoirs of S. japonicum, particularly in areas with low to high infection intensity, or where, following control efforts, there is suspected elimination of schistosomiasis japonica.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.