ArticlePloS one2012
Wolbachia-induced aae-miR-12 miRNA negatively regulates the expression of MCT1 and MCM6 genes in Wolbachia-infected mosquito cell line.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- microRNA profiles and functions in mosquitoes.PLoS neglected tropical diseases · 2018Pooled it
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- Insights into the microRNA landscape of Rhodnius prolixus, a vector of Chagas disease.Scientific reports · 2023Article
- Small RNA sequencing of field Culex mosquitoes identifies patterns of viral infection and the mosquito immune response.Scientific reports · 2023Article
- Aedes mosquitoes in the emerging threat of urban yellow fever transmission.Reviews in medical virology · 2022Review
- An Insight Into the microRNA Profile of the Ectoparasitic MiteFrontiers in cellular and infection microbiology · 2022Article
- miRNAs of Aedes aegypti (Linnaeus 1762) conserved in six orders of the class Insecta.Scientific reports · 2021Article
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- Intracellular Interactions Between Arboviruses andFrontiers in cellular and infection microbiology · 2021Review
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
- How do Wolbachia modify the Drosophila ovary? New evidences support the "titration-restitution" model for the mechanisms of Wolbachia-induced CI.BMC genomics · 2019Article
- Identification ofFrontiers in physiology · 2019Article
- miRNAs in Insects Infected by Animal and Plant Viruses.Viruses · 2018Review
- Conflict in the Intracellular Lives of Endosymbionts and Viruses: A Mechanistic Look at Wolbachia-Mediated Pathogen-blocking.Viruses · 2018Review
- Comparative expression profile of microRNAs in Anopheles anthropophagus midgut after blood-feeding and Plasmodium infection.Parasites & vectors · 2017Article
- Wolbachia Blocks Viral Genome Replication Early in Infection without a Transcriptional Response by the Endosymbiont or Host Small RNA Pathways.PLoS pathogens · 2016Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBest recognized for its role in manipulating host reproduction, the parasitic gram-negative Wolbachia pipientis is known to colonize a wide range of invertebrates. The endosymbiotic bacterium has recently been shown to cause a life-shortening effect as well as inhibiting replication of arboviruses in Aedes aegypti; although the molecular mechanisms behind these effects are largely unknown. MicroRNAs (miRNAs) have been determined to have a wide range of roles in regulating gene expression in eukaryotes. A recent study showed that several A. aegypti mosquito miRNAs are differentially expressed when infected with Wolbachia. METHODOLOGY/PRINCIPAL
findingsBased on the prior knowledge that one of these miRNAs, aae-miR-12, is differentially expressed in mosquitoes infected with Wolbachia, we aimed to determine any significance of this mediation. We also set out to characterize the target genes of this miRNA in the A. aegpyti genome. Bioinformatic approaches predicted a list of potential target genes and subsequent functional analyses confirmed that two of these, DNA replication licensing (MCM6) and monocarboxylate transporter (MCT1), are under the regulative control of aae-miR-12. We also demonstrated that aae-miR-12 is critical in the persistence of Wolbachia in the host cell. CONCLUSIONS/SIGNIFICANCE: Our study has identified two target genes of aae-miR-12, a differentially expressed mosquito miRNA in Wolbachia-infected cells, and determined that the miRNA affects Wolbachia density in the host cells.
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