ArticlePloS one2012
Hypoxia increases mouse satellite cell clone proliferation maintaining both in vitro and in vivo heterogeneity and myogenic potential.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.
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Who cites it
23 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Hypoxic Signaling in Skeletal Muscle Maintenance and Regeneration: A Systematic Review.Frontiers in physiology · 2021Pooled it
- Glutamine-driven reductive TCA cycle metabolism supports aged muscle stem cell function via de novo lipogenesis.Nature aging · 2026Article
- Transient hypoxia followed by progressive reoxygenation is required for muscle repair.EMBO reports · 2026Article
- Effects of culture temperature (37°C, 39°C) and oxygen concentration (20%, 2%) on proliferation and differentiation of C2C12 cells.Journal of animal science and technology · 2025Article
- A Glance into the Near Future: Cultivated Meat from Mammalian and Insect Cells.Small science · 2024Article
- Methods to Isolate Muscle Stem Cells for Cell-Based Cultured Meat Production: A Review.Animals : an open access journal from MDPI · 2024Review
- Current Research, Industrialization Status, and Future Perspective of Cultured Meat.Food science of animal resources · 2024Review
- DNA-PKcs regulates myogenesis in an Akt-dependent manner independent of induced DNA damage.Cell death and differentiation · 2023Article
- Effects of Hypoxia on Proliferation and Differentiation in Belgian Blue and Hanwoo Muscle Satellite Cells for the Development of Cultured Meat.Biomolecules · 2022Article
- Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives.Biomolecules · 2022Review
- Fusion of Normoxic- and Hypoxic-Preconditioned Myoblasts Leads to Increased Hypertrophy.Cells · 2022Article
- The effect of hypoxia on myogenic differentiation and multipotency of the skeletal muscle-derived stem cells in mice.Stem cell research & therapy · 2022Article
- BMAL1 drives muscle repair through control of hypoxic NADGenes & development · 2022Article
- Hypoxia preconditioned bone marrow-derived mesenchymal stromal/stem cells enhance myoblast fusion and skeletal muscle regeneration.Stem cell research & therapy · 2021Article
- Overfeeding and Substrate Availability, But Not Age or BMI, Alter Human Satellite Cell Function.Nutrients · 2020Article
- Prolyl hydroxylase domain 2 reduction enhances skeletal muscle tissue regeneration after soft tissue trauma in mice.PloS one · 2020Article
- Muscle stem cells: what's new in orthopedics?Acta bio-medica : Atenei Parmensis · 2019Review
- Disruption of mitochondrial quality control in peripheral artery disease: New therapeutic opportunities.Pharmacological research · 2017Review
- Low Oxygen Tension Enhances Expression of Myogenic Genes When Human Myoblasts Are Activated from G0 Arrest.PloS one · 2016Article
- Endothelial properties of third-trimester amniotic fluid stem cells cultured in hypoxia.Stem cell research & therapy · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Satellite cells (SCs) are essential for postnatal muscle growth and regeneration, however, their expansion potential in vitro is limited. Recently, hypoxia has been used to enhance proliferative abilities in vitro of various primary cultures. Here, by isolating SCs from single mouse hindlimb skeletal myofibers, we were able to distinguish two subpopulations of clonally cultured SCs (Low Proliferative Clones--LPC--and High Proliferative Clones--HPC), which, as shown in rat skeletal muscle, were present at a fixed proportion. In addition, culturing LPC and HPC at a low level of oxygen we observed a two fold increased proliferation both for LPC and HPC. LPC showed higher myogenic regulatory factor (MRF) expression than HPC, particularly under the hypoxic condition. Notably, a different myogenic potential between LPC and HPC was retained in vivo: green fluorescent protein (GFP)+LPC transplantation in cardiotoxin-injured Tibialis Anterior led to a higher number of new GFP+muscle fibers per transplanted cell than GFP+HPC. Interestingly, the in vivo myogenic potential of a single cell from an LPC is similar if cultured both in normoxia and hypoxia. Therefore, starting from a single satellite cell, hypoxia allows a larger expansion of LPC than normal O(2) conditions, obtaining a consistent amount of cells for transplantation, but maintaining their myogenic regeneration potential.
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