ArticlePloS one2012
Identification of novel and differentially expressed MicroRNAs of dairy goat mammary gland tissues using solexa sequencing and bioinformatics.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 77 citations in OpenAlex.
- Review
- Dynamic Analysis of microRNAs from Different Life Stages ofPathogens (Basel, Switzerland) · 2022Article
- The Role of microRNAs in the Mammary Gland Development, Health, and Function of Cattle, Goats, and Sheep.Non-coding RNA · 2021Review
- Bta-miR-34b controls milk fat biosynthesis via the Akt/mTOR signaling pathway by targeting RAI14 in bovine mammary epithelial cells.Journal of animal science and biotechnology · 2021Article
- miRNA Regulatory Functions in Farm Animal Diseases, and Biomarker Potentials for Effective Therapies.International journal of molecular sciences · 2021Review
- Review
- Differential expression profiles of microRNAs in musk gland of unmated and mated forest musk deer (PeerJ · 2021Article
- A comparative analysis of gene expression induced by the embryo in the caprine endometrium.Veterinary medicine and science · 2020Article
- Article
- High-throughput sequencing analysis identified microRNAs associated with egg production in ducks ovaries.PeerJ · 2020Article
- Bta-miR-34b regulates milk fat biosynthesis by targeting mRNA decapping enzyme 1A (DCP1A) in cultured bovine mammary epithelial cells1.Journal of animal science · 2019Article
- Deep RNA-Seq reveals miRNome differences in mammary tissue of lactating Holstein and Montbéliarde cows.BMC genomics · 2019Article
- Transcriptome and Proteome Alternation With Resistance toFrontiers in physiology · 2019Article
- Characterization of miRNA profiles in the mammary tissue of dairy cattle in response to heat stress.BMC genomics · 2018Article
- Identification and characterization of microRNA in the lung tissue of pigs with different susceptibilities to PCV2 infection.Veterinary research · 2018Article
- High-throughput sequencing analysis of differentially expressed miRNAs and target genes in ischemia/reperfusion injury and apelin-13 neuroprotection.Neural regeneration research · 2018Article
- Screening of miRNA profiles and construction of regulation networks in early and late lactation of dairy goat mammary glands.Scientific reports · 2017Article
- Detection and comparison of microRNAs in the caprine mammary gland tissues of colostrum and common milk stages.BMC genetics · 2017Article
- miR-2478 inhibits TGFβ1 expression by targeting the transcriptional activation region downstream of the TGFβ1 promoter in dairy goats.Scientific reports · 2017Article
- Sunflower oil supplementation affects the expression of miR-20a-5p and miR-142-5p in the lactating bovine mammary gland.PloS one · 2017Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs are small, noncoding RNA molecules that regulate gene expression at the post-transcriptional level and play an important role in various biological processes. Although most microRNAs expression profiles studies have been performed in humans or rodents, relatively limited knowledge also exists in other mammalian species. The identification of the full repertoire of microRNAs expressed in the lactating mammary gland of Capra hircus would significantly increase our understanding of the physiology of lactating mammary glands. In this study, two libraries were constructed using the lactating mammary gland tissues of Laoshan dairy goats (Capra hircus) during peak and late lactation. Solexa high-throughput sequencing technique and bioinformatics were used to determine the abundance and differential expression of the microRNAs between peak and late lactation. As a result, 19,044,002 and 7,385,833 clean reads were obtained, respectively, and 1,113 conserved known microRNAs and 31 potential novel microRNA candidates were identified. A total of 697 conserved microRNAs were significantly differentially expressed with a P-value<0.01, 272 microRNAs were up-regulated and 425 microRNAs were down-regulated during peak lactation. The results were validated using real-time quantitative RT-PCR. 762,557 annotated mRNA transcripts were predicted as putative target gene candidates. The GO annotation and KEGG pathway analysis suggested that differentially expressed microRNAs were involved in mammary gland physiology, including signal transduction, and cell-cell and cell-extracellular communications. This study provided the first global of the microRNA in Capra hircus and expanded the repertoire of microRNAs. Our results have great significance and value for the elucidation of complex regulatory networks between microRNAs and mRNAs and for the study of mammary gland physiology and lactation.
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