Evidence map›Paper›PMID 23166612›Full record

ArticlePloS one2012

A U-Box E3 ubiquitin ligase, PUB20, interacts with the Arabidopsis G-protein β subunit, AGB1.

Shio Kobayashi, Daisuke Tsugama, Shenkui Liu, Tetsuo Takano

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.7field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
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  6. The G ProteinPlant physiology · 2018
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Shio KobayashiAsian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo, Japan.
Daisuke Tsugama
Shenkui Liu
Tetsuo Takano
The University of Tokyo · JPNortheast Forestry University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An Arabidopsis U-box E3 ubiquitin ligase Plant U-box 20 (PUB20; alternatively called AtCMPG1) was identified as a possible interactor of the Arabidopsis G-protein β subunit, AGB1, by yeast two-hybrid screening. A bimolecular fluorescence complementation (BiFC) assay showed that PUB20 interacted with AGB1 in the nuclei and the cytosol. The expression levels of PUB20 and its closest homolog, PUB21 were stable under many conditions. GUS driven by the PUB20 promoter was active in anthers, pollen, premature seeds and receptacles and GUS driven by the PUB21 promoter was active in anthers and funiculi. PUB20 was found to have autoubiquitination activity in vitro.

Indexed as

ArabidopsisArabidopsis ProteinsDNA PrimersGTP-Binding Protein beta SubunitsPromoter Regions, GeneticReverse Transcriptase Polymerase Chain ReactionTwo-Hybrid System TechniquesUbiquitinationUbiquitin-Protein LigasesAGB1 protein, ArabidopsisArabidopsis ProteinsDNA PrimersGTP-Binding Protein beta SubunitsUbiquitin-Protein Ligases

Identifiers

PMID23166612
PMCPMC3499536
OpenAlexW1986264232

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.