ArticlePlant physiology2013
Metabolic engineering of tomato fruit organic acid content guided by biochemical analysis of an introgression line.
Article in Plant physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- Integrated transcriptome-metabolome analyses reveal regulatory networks underlying soluble solids accumulation in Capsicum chinense fruits.The Plant journal : for cell and molecular biology · 2026Article
- Signaling mechanisms governing the environmental modulation of fruit quality development.Horticulture research · 2026Article
- Molecular orchestration of malate and citrate metabolism: regulatory networks governing organic acid dynamics and fruit quality attributes.Horticulture research · 2026Article
- Recent Advances in Studying the Regulation of Fruit Ripening in Tomato Using Genetic Engineering Approaches.International journal of molecular sciences · 2024Review
- A pear S1-bZIP transcription factor PpbZIP44 modulates carbohydrate metabolism, amino acid, and flavonoid accumulation in fruits.Horticulture research · 2023Article
- Changes and response mechanism of sugar and organic acids in fruits under water deficit stress.PeerJ · 2022Review
- Beyond the limits of photoperception: constitutively active PHYTOCHROME B2 overexpression as a means of improving fruit nutritional quality in tomato.Plant biotechnology journal · 2020Article
- Fruit metabolic and transcriptional programs differentiate among Andean tomato (Solanum lycopersicum L.) accessions.Planta · 2019Article
- Quantification of sugars and organic acids in tomato fruits.MethodsX · 2018Article
- Gene coexpression network analysis of fruit transcriptomes uncovers a possible mechanistically distinct class of sugar/acid ratio-associated genes in sweet orange.BMC plant biology · 2017Article
- Citrus CitNAC62 cooperates with CitWRKY1 to participate in citric acid degradation via up-regulation of CitAco3.Journal of experimental botany · 2017Article
- The Unusual Acid-Accumulating Behavior during Ripening of Cherimoya (Annona cherimola Mill.) is Linked to Changes in Transcription and Enzyme Activity Related to Citric and Malic Acid Metabolism.Molecules (Basel, Switzerland) · 2016Article
- Exploring tomato Solanum pennellii introgression lines for residual biomass and enzymatic digestibility traits.BMC genetics · 2016Article
- Nitric Oxide Overproduction in TomatoFrontiers in plant science · 2016Article
- Low Temperature Induced Changes in Citrate Metabolism in Ponkan (Citrus reticulata Blanco cv. Ponkan) Fruit during Maturation.PloS one · 2016Article
- Integrated Systems Biology Analysis of Transcriptomes Reveals Candidate Genes for Acidity Control in Developing Fruits of Sweet Orange (Citrus sinensis L. Osbeck).Frontiers in plant science · 2016Article
- A tonoplast Glu/Asp/GABA exchanger that affects tomato fruit amino acid composition.The Plant journal : for cell and molecular biology · 2015Article
- Evolution and functional implications of the tricarboxylic acid cycle as revealed by phylogenetic analysis.Genome biology and evolution · 2014Article
- Plant metabolic modeling: achieving new insight into metabolism and metabolic engineering.The Plant cell · 2014Review
- Remarkable reproducibility of enzyme activity profiles in tomato fruits grown under contrasting environments provides a roadmap for studies of fruit metabolism.Plant physiology · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Organic acid content is regarded as one of the most important quality traits of fresh tomato (Solanum lycopersicum). However, the complexity of carboxylic acid metabolism and storage means that it is difficult to predict the best way to engineer altered carboxylic acid levels. Here, we used a biochemical analysis of a tomato introgression line with increased levels of fruit citrate and malate at breaker stage to identify a metabolic engineering target that was subsequently tested in transgenic plants. Increased carboxylic acid levels in introgression line 2-5 were not accompanied by changes in the pattern of carbohydrate oxidation by pericarp discs or the catalytic capacity of tricarboxylic acid cycle enzymes measured in isolated mitochondria. However, there was a significant decrease in the maximum catalytic activity of aconitase in total tissue extracts, suggesting that a cytosolic isoform of aconitase was affected. To test the role of cytosolic aconitase in controlling fruit citrate levels, we analyzed fruit of transgenic lines expressing an antisense construct against SlAco3b, one of the two tomato genes encoding aconitase. A green fluorescent protein fusion of SlAco3b was dual targeted to cytosol and mitochondria, while the other aconitase, SlAco3a, was exclusively mitochondrial when transiently expressed in tobacco (Nicotiana tabacum) leaves. Both aconitase transcripts were decreased in fruit from transgenic lines, and aconitase activity was reduced by about 30% in the transgenic lines. Other measured enzymes of carboxylic acid metabolism were not significantly altered. Both citrate and malate levels were increased in ripe fruit of the transgenic plants, and as a consequence, total carboxylic acid content was increased by 50% at maturity.
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