ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2013
Real-time cell viability assays using a new anthracycline derivative DRAQ7®.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Src-dependent tyrosine-phosphorylation of NM2A has a protective role against bacterial pore-forming toxins.PLoS pathogens · 2026Article
- DRAQ7 overcomes the complications associated with autofluorescence of doxorubicin during flow cytometric analyses.Biology methods & protocols · 2026Article
- More than just life and death: advances in imaging and analysis for 3D-bioprinted tissues.Frontiers in bioengineering and biotechnology · 2025Article
- Engineering Endosymbiotic Growth ofACS synthetic biology · 2022Article
- Comprehensive Flow-Cytometric Quality Assessment of Ram Sperm Intended for Gene Banking Using Standard and Novel Fertility Biomarkers.International journal of molecular sciences · 2022Article
- Phenotypic Screening for Small Molecules that Protect β-Cells from Glucolipotoxicity.ACS chemical biology · 2022Article
- Natural Compounds as Target Biomolecules in Cellular Adhesion and Migration: From Biomolecular Stimulation to Label-Free Discovery and Bioactivity-Based Isolation.Biomedicines · 2021Review
- Pseudomonas syringae pv.mBio · 2021Article
- Imaging-Based Machine Learning Analysis of Patient-Derived Tumor Organoid Drug Response.Frontiers in oncology · 2021Article
- DRAQ7 as an Alternative to MTT Assay for Measuring Viability of Glioma Cells Treated With Polyphenols.Anticancer research · 2020Article
- Immune lymphocytes halt replication of Francisella tularensis LVS within the cytoplasm of infected macrophages.Scientific reports · 2020Article
- Targeting protein homeostasis with nelfinavir/salinomycin dual therapy effectively induces death of mTORC1 hyperactive cells.Oncotarget · 2017Article
- Investigation of terahertz radiation influence on rat glial cells.Biomedical optics express · 2017Article
- Robust high-throughput kinetic analysis of apoptosis with real-time high-content live-cell imaging.Cell death & disease · 2016Article
- Wrinkled, wavelength-tunable graphene-based surface topographies for directing cell alignment and morphology.Carbon · 2016Article
- Evaluation of impermeant, DNA-binding dye fluorescence as a real-time readout of eukaryotic cell toxicity in a high throughput screening format.Assay and drug development technologies · 2014Article
- Microflow cytometers with integrated hydrodynamic focusing.Sensors (Basel, Switzerland) · 2013Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 5 countries.
Funding
Abstract
The exclusion of charged fluorescent dyes by intact cells has become a well-established assay for determining viability of cells. In search for a noninvasive fluorescent probe capable of long-term monitoring of cell death in real-time, we evaluated a new anthracycline derivative DRAQ7. The novel probe does not penetrate the plasma membrane of living cells but when the membrane integrity is compromised, it enters and binds readily to nuclear DNA to report cell death. It proved to be nontoxic to a panel of cancer cell lines grown continuously for up to 72 h and did not induce any detectable DNA damage signaling when analyzed using laser scanning microscopy and flow cytometry. The DRAQ7 provided a sensitive, real-time readout of cell death induced by a variety of stressors such as hypoxia, starvation, and drug-induced cytotoxicity. The overall responses to anticancer agents and resulting pharmacological dose-response profiles were not affected by the growth of tumor cells in the presence DRAQ7. Moreover, we for the first time introduced a near real-time microflow cytometric assay based on combination of DRAQ7 and mitochondrial inner membrane potential (ΔΨ(m) ) sensitive probe TMRM. We provide evidence that this low-dosage, real-time labeling procedure provides multiparameter and kinetic fingerprint of anticancer drug action.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.