ArticleBMC microbiology2012
Inter- and intra-subtype genotypic differences that differentiate Mycobacterium avium subspecies paratuberculosis strains.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Mycobacterium avium subsp. paratuberculosis (MAP) molecular diversity in cattle, sheep, and goats from Latin America and the Caribbean: a systematic review.Tropical animal health and production · 2021Pooled it
- East AsianJournal of veterinary science · 2026Article
- High Molecular Diversity ofInternational journal of molecular sciences · 2025Article
- Genetic and chemical control of tuberculostearic acid production inMicrobiology spectrum · 2024Article
- Molecular Diversity ofAnimals : an open access journal from MDPI · 2023Article
- The pan-genome of Mycobacterium avium subsp. paratuberculosis (Map) confirms ancestral lineage and reveals gene rearrangements within Map Type S.BMC genomics · 2023Article
- InvestigatingMicrobiology spectrum · 2023Article
- Genomic epidemiology ofFrontiers in genetics · 2023Article
- Strain diversity inFrontiers in veterinary science · 2023Article
- Phylogenetic and Multiple-Locus Variable number tandem repeat analysis of Mycobacterium avium subsp. paratuberculosis isolates from Argentina.Veterinary research communications · 2022Article
- A Comparative Study on the Efficiency of TwoVaccines · 2021Article
- Molecular characterisation of Mycobacterium avium subsp. paratuberculosis in Australia.BMC microbiology · 2021Article
- Comparative Genomics ofFrontiers in veterinary science · 2021Article
- Engineering Synthetic Lipopeptide Antigen for Specific Detection ofFrontiers in veterinary science · 2021Article
- Whole-Genome Analysis ofFrontiers in microbiology · 2021Article
- Molecular characterization ofCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2019Article
- Morphological and Molecular Characterization of a NewFrontiers in veterinary science · 2019Article
- Genotyping methods and molecular epidemiology ofInternational journal of veterinary science and medicine · 2018Review
- EnvironmentalFrontiers in cellular and infection microbiology · 2018Article
- Phylogenomic exploration of the relationships between strains of Mycobacterium avium subspecies paratuberculosis.BMC genomics · 2016Article
Corrections and comments
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMycobacterium avium subspecies paratuberculosis (Map) is the aetiological agent of Johne's disease or paratuberculosis and is included within the Mycobacterium avium complex (MAC). Map strains are of two major types often referred to as 'Sheep' or 'S-type' and 'Cattle' or 'C-type'. With the advent of more discriminatory typing techniques it has been possible to further classify the S-type strains into two groups referred to as Type I and Type III. This study was undertaken to genotype a large panel of S-type small ruminant isolates from different hosts and geographical origins and to compare them with a large panel of well documented C-type isolates to assess the genetic diversity of these strain types. Methods used included Mycobacterial Interspersed Repetitive Units - Variable-Number Tandem Repeat analysis (MIRU-VNTR), analysis of Large Sequence Polymorphisms by PCR (LSP analysis), Single Nucleotide Polymorphism (SNP) analysis of gyr genes, Pulsed-Field Gel Electrophoresis (PFGE) and Restriction Fragment Length Polymorphism analysis coupled with hybridization to IS900 (IS900-RFLP) analysis.
resultsThe presence of LSP(A)4 and absence of LSP(A)20 was confirmed in all 24 Map S-type strains analysed. SNPs within the gyr genes divided the S-type strains into types I and III. Twenty four PFGE multiplex profiles and eleven different IS900-RFLP profiles were identified among the S-type isolates, some of them not previously published. Both PFGE and IS900-RFLP segregated the S-type strains into types I and III and the results concurred with those of the gyr SNP analysis. Nine MIRU-VNTR genotypes were identified in these isolates. MIRU-VNTR analysis differentiated Map strains from other members of Mycobacterium avium Complex, and Map S-type from C-type but not type I from III. Pigmented Map isolates were found of type I or III.
conclusionThis is the largest panel of S-type strains investigated to date. The S-type strains could be further divided into two subtypes, I and III by some of the typing techniques (IS900-RFLP, PFGE and SNP analysis of the gyr genes). MIRU-VNTR did not divide the strains into the subtypes I and III but did detect genetic differences between isolates within each of the subtypes. Pigmentation is not exclusively associated with type I strains.
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