ArticleJournal of ovarian research2012
Markers of stem cells in human ovarian granulosa cells: is there a clinical significance in ART?
Article in Journal of ovarian research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Female Age Affects the Mesenchymal Stem Cell Characteristics of Aspirated Follicular Cells in the In Vitro Fertilization Programme.Stem cell reviews and reports · 2019Trial
- AKT, p-AKT, ERK1/2 and p-ERK1/2 in Mural Granulosa Cells Are Not Correlated to Different Ovarian Stimulation Protocols in Patients Undergoing Assisted Reproductive Treatment.Life (Basel, Switzerland) · 2024Article
- Review
- Combination of Estradiol with Leukemia Inhibitory Factor Stimulates Granulosa Cells Differentiation into Oocyte-Like Cells.Advanced pharmaceutical bulletin · 2021Article
- Stratifying Cumulus Cell Samples Based on Molecular Profiling to Help Resolve Biomarker Discrepancies and to Predict Oocyte Developmental Competence.International journal of molecular sciences · 2021Article
- Characterization of stem cells from human ovarian follicular fluid; a potential source of autologous stem cell for cell-based therapy.Human cell · 2021Article
- Reprogramming of Ovarian Granulosa Cells by YAP1 Leads to Development of High-Grade Cancer with Mesenchymal Lineage and Serous Features.Science bulletin · 2020Article
- Transcriptomic analysis of expression of genes regulating cell cycle progression in porcine ovarian granulosa cells during short-term in vitro primary culture.Histochemistry and cell biology · 2020Article
- In focus in HCB.Histochemistry and cell biology · 2020Article
- Evaluation of stemness marker expression in bovine ovarian granulosa cells.Animal reproduction · 2019Article
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Characterization and identification of human immortalized granulosa cells derived from ovarian follicular fluid.Experimental and therapeutic medicine · 2019Article
- Ovarian regeneration: The potential for stem cell contribution in the postnatal ovary to sustained endocrine function.Molecular and cellular endocrinology · 2017Review
- Changes in the expression of OCT4 in mouse ovary during estrous cycle.Veterinary research forum : an international quarterly journal · 2017Article
- Granulosa cell-derived induced pluripotent stem cells exhibit pro-trophoblastic differentiation potential.Stem cell research & therapy · 2015Article
- Identification and characterization of epithelial cells derived from human ovarian follicular fluid.Stem cell research & therapy · 2015Article
- Self-Organizing Feature Maps Identify Proteins Critical to Learning in a Mouse Model of Down Syndrome.PloS one · 2015Article
- Expression of mesenchymal stem cells-related genes and plasticity of aspirated follicular cells obtained from infertile women.BioMed research international · 2014Article
- A comparison of the multiple oocyte maturation gene expression patterns between the newborn and adult mouse ovary.Iranian journal of reproductive medicine · 2013Article
- Plasticity of granulosa cells: on the crossroad of stemness and transdifferentiation potential.Journal of assisted reproduction and genetics · 2013Review
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelled
backgroundThe purpose of the study was to determine the incidence of gene expression of Oct-4 and DAZL, which are typical markers for stem cells, in human granulosa cells during ovarian stimulation in women with normal FSH levels undergoing IVF or ICSI and to discover any clinical significance of such expression in ART.
methodsTwenty one women underwent ovulation induction for IVF or ICSI and ET with standard GnRH analogue-recombinant FSH protocol. Infertility causes were male and tubal factor. Cumulus-mature oocyte complexes were denuded separately and granulosa cells were analyzed for each patient separately using quantitative reverse-transcription-polymerase chain reaction analysis for Oct-4 and DAZL gene expression with G6PD gene as internal standard.
resultsG6PD and Oct-4 mRNA was detected in the granulosa cells in 47.6% (10/21). The median of Oct-4 mRNA/G6PD mRNA was 1.75 with intra-quarteral range from 0.10 to 98.21. The OCT-4 mRNA expression was statistically significantly correlated with the number of oocytes retrieved; when the Oct-4 mRNA expression was higher, then more than six oocytes were retrieved (p=0.037, Wilcoxon rank-sum). No detection of DAZL mRNA was found in granulosa cells. There was no additional statistically significant correlation between the levels of Oct-4 expression and FSH basal levels or estradiol peak levels or dosage of FSH for ovulation induction. No association was found between the presence or absence of Oct-4 mRNA expression in granulosa cells and ovarian response to gonadotropin stimulation. Also, no influence on pregnancy was observed between the presence or absence of Oct-4 mRNA expression in granulosa cells or to its expression levels accordingly.
conclusionsExpression of OCT-4 mRNA, which is a typical stem cell marker and absence of expression of DAZL mRNA, which is a typical germ cell marker, suggest that a subpopulation of luteinized granulosa cells in healthy ovarian follicles (47.6%) consists of stem cells, which are not originated from primordial germ cells. Absence of Oct-4 gene expression in more than half of the cases means probably the end of the productive journey of these cells, towards the oocyte.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.