ArticleScientific reports2012
DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA.
Article in Scientific reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed.
- Nucleic acid aptamers in orthopedic diseases: promising therapeutic agents for bone disorders.Bone research · 2025Review
- Recent Progress in DNA Biosensors: Target-Specific and Structure-Guided Signal Amplification.Biosensors · 2025Review
- Nucleic Acid Framework-Enabled Spatial Organization for Biological Applications.Chem & bio engineering · 2025Review
- Label-free, ultra-low detection limit DNA biosensor using high quality optical microcavity functionalized by DNA tetrahedral nanostructure probes.Nanophotonics (Berlin, Germany) · 2023Article
- Nucleic acid nanoassembly-enhanced RNA therapeutics and diagnosis.Acta pharmaceutica Sinica. B · 2023Review
- Designer tetrahedral DNA framework-based microfluidic technology for multivalent capture and release of circulating tumor cells.Materials today. Bio · 2022Article
- Ultrasensitive PCR-Free detection of whole virus genome by electrochemiluminescence.Biosensors & bioelectronics · 2022Article
- Article
- Development of Electrochemical Biosensor for miR204-Based Cancer Diagnosis.Interdisciplinary sciences, computational life sciences · 2022Article
- MoSBioactive materials · 2021Review
- The protective effect of tetrahedral framework nucleic acids on periodontium under inflammatory conditions.Bioactive materials · 2021Article
- DNA Transformations for Diagnosis and Therapy.Advanced functional materials · 2021Review
- A controlled T7 transcription-driven symmetric amplification cascade machinery for single-molecule detection of multiple repair glycosylases.Chemical science · 2021Article
- DNA tetrahedron-mediated immune-sandwich assay for rapid and sensitive detection of PSA through a microfluidic electrochemical detection system.Microsystems & nanoengineering · 2021Article
- Electrochemical DNA Sensor for Sensitive BRCA1 Detection Based on DNA Tetrahedral-Structured Probe and Poly-Adenine Mediated Gold Nanoparticles.Biosensors · 2020Article
- Tetrahedral framework nucleic acids promote scarless healing of cutaneous wounds via the AKT-signaling pathway.Signal transduction and targeted therapy · 2020Article
- DNA Microsystems for Biodiagnosis.Micromachines · 2020Review
- Single Functionalized pRNA/Gold Nanoparticle for Ultrasensitive MicroRNA Detection Using Electrochemical Surface-Enhanced Raman Spectroscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- DNA nanotechnology approaches for microRNA detection and diagnosis.Nucleic acids research · 2019Review
- Bioapplications of DNA nanotechnology at the solid-liquid interface.Chemical Society reviews · 2019Review
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) have been identified as promising cancer biomarkers due to their stable presence in serum. As an alternative to PCR-based homogenous assays, surface-based electrochemical biosensors offer great opportunities for low-cost, point-of-care tests (POCTs) of disease-associated miRNAs. Nevertheless, the sensitivity of miRNA sensors is often limited by mass transport and crowding effects at the water-electrode interface. To address such challenges, we herein report a DNA nanostructure-based interfacial engineering approach to enhance binding recognition at the gold electrode surface and drastically improve the detection sensitivity. By employing this novel strategy, we can directly detect as few as attomolar (<1, 000 copies) miRNAs with high single-base discrimination ability. Given that this ultrasensitive electrochemical miRNA sensor (EMRS) is highly reproducible and essentially free of prior target labeling and PCR amplification, we also demonstrate its application by analyzing miRNA expression levels in clinical samples from esophageal squamous cell carcinoma (ESCC) patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.