ArticleFrontiers in plant science2012
The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression.
Article in Frontiers in plant science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 56 citations in OpenAlex.
- Article
- OsNAC121 regulates root development, tillering, panicle morphology, and grain filling in rice plant.Plant molecular biology · 2024Article
- Should I stay or should I go? Trafficking of plant extra-nuclear transcription factors.The Plant cell · 2024Review
- Detection of Stress-Induced Changes in Subcellular Protein Distribution.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Thiol Redox Proteomics for Identifying Redox-Sensitive Cysteine Residues Within the Protein of Interest During Stress.Methods in molecular biology (Clifton, N.J.) · 2024Article
- How to Cope with the Challenges of Environmental Stresses in the Era of Global Climate Change: An Update on ROS Stave off in Plants.International journal of molecular sciences · 2022Review
- Proteolytic Activation of Plant Membrane-Bound Transcription Factors.Frontiers in plant science · 2022Review
- MAG2 and MAL Regulate Vesicle Trafficking and Auxin Homeostasis With Functional Redundancy.Frontiers in plant science · 2022Article
- Redox feedback regulation of ANAC089 signaling alters seed germination and stress response.Cell reports · 2021Article
- Signaling Toward Reactive Oxygen Species-Scavenging Enzymes in Plants.Frontiers in plant science · 2020Review
- Multiple downy mildew effectors target the stress-related NAC transcription factor LsNAC069 in lettuce.The Plant journal : for cell and molecular biology · 2019Article
- Maintaining homeostasis by controlled alternatives for energy distribution in plant cells under changing conditions of supply and demand.Photosynthesis research · 2019Article
- Activation of the Transducers of Unfolded Protein Response in Plants.Frontiers in plant science · 2018Review
- Evolutionary and Functional Analysis of Membrane-Bound NAC Transcription Factor Genes in Soybean.Plant physiology · 2016Article
- Article
- The ABI4-induced Arabidopsis ANAC060 transcription factor attenuates ABA signaling and renders seedlings sugar insensitive when present in the nucleus.PLoS genetics · 2014Article
- The membrane-associated transcription factor NAC089 controls ER-stress-induced programmed cell death in plants.PLoS genetics · 2014Article
- Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells.Frontiers in plant science · 2013Review
- Retrograde signals galore.Frontiers in plant science · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The stromal ascorbate peroxidase (sAPX) functions as central element of the chloroplast antioxidant defense system. Its expression is under retrograde control of chloroplast signals including redox- and reactive oxygen species-linked cues. The sAPX promoter of Arabidopsis thaliana was dissected in transient reporter assays using mesophyll protoplasts. The study revealed regulatory elements up to -1868 upstream of the start codon. By yeast-one-hybrid screening, the transcription factor ANAC089 was identified to bind to the promoter fragment 2 (-1262 to -1646 bp upstream of translational initiation). Upon mutation of the cis-acting element CACG, binding of ANAC089 was abolished. Expression of a fused fluorescent protein version and comparison with known endomembrane markers localized ANAC089 to the trans-Golgi network and the ER. The transcription factor was released upon treatment with reducing agents and targeted to the nucleus. Transactivation assays using wild type and mutated versions of the promoter showed a partial suppression of reporter expression. The data indicate that ANAC089 functions in a negative retrograde loop, lowering sAPX expression if the cell encounters a highly reducing condition. This conclusion was supported by reciprocal transcript accumulation of ANAC089 and sAPX during acclimation to low, normal, and high light conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.