ArticleFrontiers in plant science2012
Sequencing of chloroplast genome using whole cellular DNA and solexa sequencing technology.
Article in Frontiers in plant science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Pan-Plastome of Greater Yam (International journal of molecular sciences · 2023Article
- Three Parts of the Plant Genome: On the Way to Success in the Production of Recombinant Proteins.Plants (Basel, Switzerland) · 2022Review
- rKOMICS: an R package for processing mitochondrial minicircle assemblies in population-scale genome projects.BMC bioinformatics · 2021Article
- Genomics Armed With Diversity Leads the Way inFrontiers in genetics · 2021Review
- Methods of analysis of chloroplast genomes of CPlant methods · 2020Article
- Evolution of plastid genomes of Holcoglossum (Orchidaceae) with recent radiation.BMC evolutionary biology · 2019Article
- Transcription is a major driving force for plastid genome instability in Arabidopsis.PloS one · 2019Article
- Characterization of the Chloroplast Genome Sequence ofMolecules (Basel, Switzerland) · 2018Article
- A missense mutation of plastid RPS4 is associated with chlorophyll deficiency in Chinese cabbage (Brassica campestris ssp. pekinensis).BMC plant biology · 2018Article
- Study of spontaneous mutations in the transmission of poplar chloroplast genomes from mother to offspring.BMC genomics · 2018Article
- Insights into population structure of East African sweetpotato cultivars from hybrid assembly of chloroplast genomes.Gates open research · 2018Article
- Article
- Genetic variation architecture of mitochondrial genome reveals the differentiation in Korean landrace and weedy rice.Scientific reports · 2017Article
- NOVOPlasty: de novo assembly of organelle genomes from whole genome data.Nucleic acids research · 2017Article
- A Phylogenetic Analysis of Chloroplast Genomes Elucidates the Relationships of the Six Economically ImportantFrontiers in plant science · 2017Article
- Rice Chloroplast Genome Variation Architecture and Phylogenetic Dissection in Diverse Oryza Species Assessed by Whole-Genome Resequencing.Rice (New York, N.Y.) · 2016Article
- Chloroplast genomes: diversity, evolution, and applications in genetic engineering.Genome biology · 2016Review
- Organelle DNA rearrangement mapping reveals U-turn-like inversions as a major source of genomic instability in Arabidopsis and humans.Genome research · 2015Article
- Plastome organization and evolution of chloroplast genes in Cardamine species adapted to contrasting habitats.BMC genomics · 2015Article
- Chloroplast genome of Aconitum barbatum var. puberulum (Ranunculaceae) derived from CCS reads using the PacBio RS platform.Frontiers in plant science · 2015Article
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7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequencing of the chloroplast (cp) genome using traditional sequencing methods has been difficult because of its size (>120 kb) and the complicated procedures required to prepare templates. To explore the feasibility of sequencing the cp genome using DNA extracted from whole cells and Solexa sequencing technology, we sequenced whole cellular DNA isolated from leaves of three Brassicarapa accessions with one lane per accession. In total, 246, 362, and 361 Mb sequence data were generated for the three accessions Chiifu-401-42, Z16, and FT, respectively. Micro-reads were assembled by reference-guided assembly using the cpDNA sequences of B. rapa, Arabidopsis thaliana, and Nicotiana tabacum. We achieved coverage of more than 99.96% of the cp genome in the three tested accessions using the B. rapa sequence as the reference. When A. thaliana or N. tabacum sequences were used as references, 99.7-99.8 or 95.5-99.7% of the B. rapa cp genome was covered, respectively. These results demonstrated that sequencing of whole cellular DNA isolated from young leaves using the Illumina Genome Analyzer is an efficient method for high-throughput sequencing of cp genome.
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