ArticleAmerican journal of physiology. Lung cellular and molecular physiology2013
Automated measurement of blood flow velocity and direction and hemoglobin oxygen saturation in the rat lung using intravital microscopy.
Article in American journal of physiology. Lung cellular and molecular physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- In vitro vascularization improves in vivo functionality of human engineered cardiac tissues.Acta biomaterialia · 2026Article
- Methods to localize cell specific alterations within the 3D context of the lung.Frontiers in physiology · 2026Review
- Hyperspectral wide-field-of-view imaging to study dynamic microcirculatory changes during hypoxia.American journal of physiology. Heart and circulatory physiology · 2022Article
- Acoustic Trauma Causes Cochlear Pericyte-to-Myofibroblast-Like Cell Transformation and Vascular Degeneration, and Transplantation of New Pericytes Prevents Vascular Atrophy.The American journal of pathology · 2020Article
- A simple automated method for continuous fieldwise measurement of microvascular hemodynamics.Microvascular research · 2019Article
- [Effect of simulated microgravity on peripheral oxygen saturation in rats].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2018Article
- A permanent window for the murine lung enables high-resolution imaging of cancer metastasis.Nature methods · 2018Article
- Measuring blood oxygen saturation along a capillary vessel in human.Biomedical optics express · 2017Article
- Article
- Effects of high-dose microbeam irradiation on tumor microvascular function and angiogenesis.Radiation research · 2015Article
- In vivo fluorescence microscopy: lessons from observing cell behavior in their native environment.Physiology (Bethesda, Md.) · 2015Review
- Microenvironmental acidosis in carcinogenesis and metastases: new strategies in prevention and therapy.Cancer metastasis reviews · 2014Review
- Automated measurement of microcirculatory blood flow velocity in pulmonary metastases of rats.Journal of visualized experiments : JoVE · 2014Article
- Anti-hypotensive treatment and endothelin blockade synergistically antagonize exercise fatigue in rats under simulated high altitude.PloS one · 2014Article
- Quantitative mapping of hemodynamics in the lung, brain, and dorsal window chamber-grown tumors using a novel, automated algorithm.Microcirculation (New York, N.Y. : 1994) · 2013Article
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Authors and funding
14 authors at 3 institutions in 1 country.
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Abstract
Intravital microscopy of the pulmonary microcirculation in research animals is of great scientific interest for its utility in identifying regional changes in pulmonary microcirculatory blood flow. Although feasibility studies have been reported, the pulmonary window can be further refined into a practical tool for pharmaceutical research and drug development. We have established a method to visualize and quantify dynamic changes in three key features of lung function: microvascular red blood cell velocity, flow direction, and hemoglobin saturation. These physiological parameters were measured in an acute closed-chest pulmonary window, which allows real-time images to be captured by fluorescence and multispectral absorption microscopy; images were subsequently quantified using computerized analysis. We validated the model by quantifying changes in microcirculatory blood flow and hemoglobin saturation in two ways: 1) after changes in inspired oxygen content and 2) after pharmacological reduction of pulmonary blood flow via treatment with the β1 adrenergic receptor blocker metoprolol. This robust and relatively simple system facilitates pulmonary intravital microscopy in laboratory rats for pharmacological and physiological research.
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