ArticleNucleic acids research2013
hnRNPL and nucleolin bind LINE-1 RNA and function as host factors to modulate retrotransposition.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 74 citations in OpenAlex.
- LINE-1 Retrotransposons and Amyotrophic Lateral Sclerosis.International journal of molecular sciences · 2026Review
- Phylogenetic relatedness rather than aquatic habitat fosters horizontal transfer of transposable elements in animals.Genome research · 2025Article
- Internal ribosome entry sites enhance translation in trans in antisense non-coding SINEUP and circular RNAs.Nucleic acids research · 2025Article
- Article
- TheViruses · 2024Article
- Retrotransposon life cycle and its impacts on cellular responses.RNA biology · 2024Review
- Sensing nucleotide composition in virus RNA.Bioscience reports · 2023Review
- Affinity-Based Interactome Analysis of Endogenous LINE-1 Macromolecules.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Long non-coding RNAeLife · 2022Article
- Condensin I and condensin II proteins form a LINE-1 dependent super condensin complex and cooperate to repress LINE-1.Nucleic acids research · 2022Article
- LINE1 are spliced in non-canonical transcript variants to regulate T cell quiescence and exhaustion.Nature genetics · 2022Article
- Review
- Depletion of nuclear LINE1 RNA in mouse ESCs and embryos.STAR protocols · 2021Article
- Binding patterns of RNA-binding proteins to repeat-derived RNA sequences reveal putative functional RNA elements.NAR genomics and bioinformatics · 2021Article
- Genomic Repeats Categorize Genes with Distinct Functions for Orchestrated Regulation.Cell reports · 2020Article
- The Nucleolin Antagonist N6L Inhibits LINE1 Retrotransposon Activity in Non-Small Cell Lung Carcinoma Cells.Journal of Cancer · 2020Article
- Comprehensive Scanning Mutagenesis of Human Retrotransposon LINE-1 Identifies Motifs Essential for Function.Genetics · 2019Article
- Poly(ADP-Ribose) Polymerase 2 Recruits Replication Protein A to Sites of LINE-1 Integration to Facilitate Retrotransposition.Molecular cell · 2019Article
- Host-transposon interactions: conflict, cooperation, and cooption.Genes & development · 2019Review
- Proteome Profile of Endogenous Retrotransposon-Associated Complexes in Human Embryonic Stem Cells.Proteomics · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Long INterspersed Element one (LINE-1, or L1), is a widely distributed, autonomous retrotransposon in mammalian genomes. During retrotransposition, L1 RNA functions first as a dicistronic mRNA and then as a template for cDNA synthesis. Previously, we defined internal ribosome entry sequences (IRESs) upstream of both ORFs (ORF1 and ORF2) in the dicistronic mRNA encoded by mouse L1. Here, RNA affinity chromatography was used to isolate cellular proteins that bind these regions of L1 RNA. Four proteins, the heterogeneous nuclear ribonucleoproteins (hnRNPs) R, Q and L, and nucleolin (NCL), appeared to interact specifically with the ORF2 IRES. These were depleted from HeLa cells to examine their effects on L1 IRES-mediated translation and L1 retrotransposition. NCL knockdown specifically reduced the ORF2 IRES activity, L1 and L1-assisted Alu retrotransposition without altering L1 RNA or protein abundance. These findings are consistent with NCL acting as an IRES trans-acting factor (ITAF) for ORF2 translation and hence a positive host factor for L1 retrotransposition. In contrast, hnRNPL knockdown dramatically increased L1 retrotransposition as well as L1 RNA and ORF1 protein, indicating that this cellular protein normally interferes with retrotransposition. Thus, hnRNPL joins a small, but growing list of cellular proteins that are potent negative regulators of L1 retrotransposition.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.