ArticleNucleic acids research2013
miRNA repression of translation in vitro takes place during 43S ribosomal scanning.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs.Life science alliance · 2026Article
- DDX6 interacts with DDX3X to repress translation in microRNA-mediated silencing.Nucleic acids research · 2025Article
- Article
- SOX9 is a target of miR-134-3p and miR-224-3p in breast cancer cell lines.Molecular and cellular biochemistry · 2023Article
- Preliminary evidence that lectins in infant soy formula apparently bind bovine milk exosomes and prevent their absorption in healthy adults.BMC nutrition · 2022Article
- Review of Ribosome Interactions with SARS-CoV-2 and COVID-19 mRNA Vaccine.Life (Basel, Switzerland) · 2022Review
- Eukaryotic initiation factor EIF-3.G augments mRNA translation efficiency to regulate neuronal activity.eLife · 2021Article
- Leveraging mRNA Sequences and Nanoparticles to Deliver SARS-CoV-2 Antigens In Vivo.Advanced materials (Deerfield Beach, Fla.) · 2020Article
- MicroRNAs: From Mechanism to Organism.Frontiers in cell and developmental biology · 2020Review
- eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR.Genome biology · 2019Article
- The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation.PLoS pathogens · 2019Article
- Migration of Small Ribosomal Subunits on the 5' Untranslated Regions of Capped Messenger RNA.International journal of molecular sciences · 2019Article
- Mechanistic Insights into MicroRNA-Mediated Gene Silencing.Cold Spring Harbor perspectives in biology · 2019Review
- A novel functional polymorphism ofAging · 2018Article
- miR-302a-5p/367-3p-HMGA2 axis regulates malignant processes during endometrial cancer development.Journal of experimental & clinical cancer research : CR · 2018Article
- PTRE-seq reveals mechanism and interactions of RNA binding proteins and miRNAs.Nature communications · 2018Article
- MicroRNAs from plants to animals, do they define a new messenger for communication?Nutrition & metabolism · 2018Review
- Translation efficiency is a determinant of the magnitude of miRNA-mediated repression.Scientific reports · 2017Article
- MicroRNAs recruit eIF4E2 to repress translation of target mRNAs.Protein & cell · 2017Article
- microRNAs stimulate translation initiation mediated by HCV-like IRESes.Nucleic acids research · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
microRNAs (miRNAs) regulate gene expression at multiple levels by repressing translation, stimulating deadenylation and inducing the premature decay of target messenger RNAs (mRNAs). Although the mechanism by which miRNAs repress translation has been widely studied, the precise step targeted and the molecular insights of such repression are still evasive. Here, we have used our newly designed in vitro system, which allows to study miRNA effect on translation independently of deadenylation. By using specific inhibitors of various stages of protein synthesis, we first show that miRNAs target exclusively the early steps of translation with no effect on 60S ribosomal subunit joining, elongation or termination. Then, by using viral proteases and IRES-driven mRNA constructs, we found that translational inhibition takes place during 43S ribosomal scanning and requires both the poly(A) binding protein and eIF4G independently from their physical interaction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.