ArticleJournal of industrial microbiology & biotechnology2013
Characterization of three new carboxylic ester hydrolases isolated by functional screening of a forest soil metagenomic library.
Article in Journal of industrial microbiology & biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 57 citations in OpenAlex.
- Bacterial and Fungal Community Responses to Long-Term Salinity Gradients in Natural Soils of Kazakhstan.Microorganisms · 2026Article
- Enzymatic degradation of PET by hydrolase from Brucella intermedia IITR130 and its genomic insights.Biodegradation · 2025Article
- An improved protocol for metagenomic DNA isolation from low microbial biomass alkaline hot-spring sediments and soil samples.3 Biotech · 2024Article
- Genome-wide analysis of lipolytic enzymes and characterization of a high-tolerant carboxylesterase fromFrontiers in microbiology · 2023Article
- Insufficiency in functional genomics studies, data, and applications: A case study of bio-prospecting research in ruminant microbiome.Frontiers in genetics · 2022Review
- Metagenomic Screening for Lipolytic Genes Reveals an Ecology-Clustered Distribution Pattern.Frontiers in microbiology · 2022Article
- Emerging priorities for microbial metagenome research.Bioresource technology reports · 2020Review
- Characterization of a novel family VIII esterase EstM2 from soil metagenome capable of hydrolyzing estrogenic phthalates.Microbial cell factories · 2020Article
- Characterization of Novel Family IV Esterase and Family I.3 Lipase from an Oil-Polluted Mud Flat Metagenome.Molecular biotechnology · 2015Article
- Biotechnological applications of functional metagenomics in the food and pharmaceutical industries.Frontiers in microbiology · 2015Review
- Metagenomics of an Alkaline Hot Spring in Galicia (Spain): Microbial Diversity Analysis and Screening for Novel Lipolytic Enzymes.Frontiers in microbiology · 2015Article
- Est10: A Novel Alkaline Esterase Isolated from Bovine Rumen Belonging to the New Family XV of Lipolytic Enzymes.PloS one · 2015Article
- Identification and characterization of a halotolerant, cold-active marine endo-β-1,4-glucanase by using functional metagenomics of seaweed-associated microbiota.Applied and environmental microbiology · 2014Article
- Two promising alkaline β-glucosidases isolated by functional metagenomics from agricultural soil, including one showing high tolerance towards harsh detergents, oxidants and glucose.Journal of industrial microbiology & biotechnology · 2014Article
- Isolation and characterization of a thermostable esterase from a metagenomic library.Journal of industrial microbiology & biotechnology · 2013Article
- Characterization of a new oxidant-stable serine protease isolated by functional metagenomics.SpringerPlus · 2013Article
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2 authors at 1 institution in 1 country.
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Abstract
Three new lipolytic genes were isolated from a forest soil metagenomic library by functional screening on tributyrin agar plates. The genes SBLip1, SBLip2 and SBLip5.1 respectively encode polypeptides of 445, 346 and 316 amino acids. Phylogenetic analyses revealed that SBLip2 and SBLip5.1 belong to bacterial esterase/lipase family IV, whereas SBLip1 shows similarity to class C β-lactamases and is thus related to esterase family VIII. The corresponding genes were overexpressed and their products purified by affinity chromatography for characterization. Analyses of substrate specificity with different p-nitrophenyl esters showed that all three enzymes have a preference for short-acyl-chain p-nitrophenyl esters, a feature of carboxylesterases as opposed to lipases. The β-lactamase activity of SBLip1, measured with the chromogenic substrate nitrocefin, was very low. The three esterases have the same optimal pH (pH 10) and remain active across a relatively broad pH range, displaying more than 60 % activity between pH 6 and 10. The temperature optima determined were 35 °C for SBLip1, 45 °C for SBLip2 and 50 °C for SBLip5.1. The three esterases displayed different levels of tolerance to salts, solvents and detergents, SBLip2 being overall more tolerant to high concentrations of solvent and SBLip5.1 less affected by detergents.
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