ArticlePhysical biology2012
Regional differences in actomyosin contraction shape the primary vesicles in the embryonic chicken brain.
Article in Physical biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 31 citations in OpenAlex.
- Multiscale Modeling of Embryonic Morphogenesis from Fertilization to Organogenesis.NPJ systems biology and applications · 2026Review
- Morphometric analysis reveals that the chick cranial neural tube expands as an active shell.bioRxiv : the preprint server for biology · 2026Article
- Biomechanical models and mechanisms of cellular morphogenesis and cerebral cortical expansion and folding.Seminars in cell & developmental biology · 2023Review
- Developmentally interdependent stretcher-compressor relationship between the embryonic brain and the surrounding scalp in the preosteogenic head.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Segmentation and patterning of the vertebrate hindbrain.Development (Cambridge, England) · 2021Review
- An energy landscape approach to understanding variety and robustness in tissue morphogenesis.Biomechanics and modeling in mechanobiology · 2020Article
- Molecular and mechanical signals determine morphogenesis of the cerebral hemispheres in the chicken embryo.Development (Cambridge, England) · 2019Article
- Formin homology 2 domain-containing 3 (Fhod3) controls neural plate morphogenesis in mouse cranial neurulation by regulating multidirectional apical constriction.The Journal of biological chemistry · 2019Article
- Evolutionary emergence of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Apoptosis generates mechanical forces that close the lens vesicle in the chick embryo.Physical biology · 2018Article
- Claudins in morphogenesis: Forming an epithelial tube.Tissue barriers · 2017Review
- A new hypothesis for foregut and heart tube formation based on differential growth and actomyosin contraction.Development (Cambridge, England) · 2017Article
- Midbrain-Hindbrain Boundary Morphogenesis: At the Intersection of Wnt and Fgf Signaling.Frontiers in neuroanatomy · 2017Review
- Contraction and stress-dependent growth shape the forebrain of the early chicken embryo.Journal of the mechanical behavior of biomedical materials · 2017Article
- Cell segregation in the vertebrate hindbrain: a matter of boundaries.Cellular and molecular life sciences : CMLS · 2015Review
- Growth and remodelling for profound circular wounds in skin.Biomechanics and modeling in mechanobiology · 2015Article
- Mechanical effects of the surface ectoderm on optic vesicle morphogenesis in the chick embryo.Journal of biomechanics · 2014Article
- Morphomechanics: transforming tubes into organs.Current opinion in genetics & development · 2014Review
- Hox proteins drive cell segregation and non-autonomous apical remodelling during hindbrain segmentation.Development (Cambridge, England) · 2014Article
- Cell segregation in the vertebrate hindbrain relies on actomyosin cables located at the interhombomeric boundaries.The EMBO journal · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
In the early embryo, the brain initially forms as a relatively straight, cylindrical epithelial tube composed of neural stem cells. The brain tube then divides into three primary vesicles (forebrain, midbrain, hindbrain), as well as a series of bulges (rhombomeres) in the hindbrain. The boundaries between these subdivisions have been well studied as regions of differential gene expression, but the morphogenetic mechanisms that generate these constrictions are not well understood. Here, we show that regional variations in actomyosin-based contractility play a major role in vesicle formation in the embryonic chicken brain. In particular, boundaries did not form in brains exposed to the nonmuscle myosin II inhibitor blebbistatin, whereas increasing contractile force using calyculin or ATP deepened boundaries considerably. Tissue staining showed that contraction likely occurs at the inner part of the wall, as F-actin and phosphorylated myosin are concentrated at the apical side. However, relatively little actin and myosin was found in rhombomere boundaries. To determine the specific physical mechanisms that drive vesicle formation, we developed a finite-element model for the brain tube. Regional apical contraction was simulated in the model, with contractile anisotropy and strength estimated from contractile protein distributions and measurements of cell shapes. The model shows that a combination of circumferential contraction in the boundary regions and relatively isotropic contraction between boundaries can generate realistic morphologies for the primary vesicles. In contrast, rhombomere formation likely involves longitudinal contraction between boundaries. Further simulations suggest that these different mechanisms are dictated by regional differences in initial morphology and the need to withstand cerebrospinal fluid pressure. This study provides a new understanding of early brain morphogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.