ArticleBiochemical and biophysical research communications2013
Insulin suppression of apolipoprotein B in McArdle RH7777 cells involves increased sortilin 1 interaction and lysosomal targeting.
Article in Biochemical and biophysical research communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Molecular Regulation and Therapeutic Targeting of VLDL Production in Cardiometabolic Disease.Cellular and molecular gastroenterology and hepatology · 2025Review
- A human iPSC-derived hepatocyte screen identifies compounds that inhibit production of Apolipoprotein B.Communications biology · 2023Article
- Effects of chlorpyrifos on non-cholinergic toxicity endpoints in immortalized and primary rat hepatocytes under normal and hepatosteatotic conditions.Toxicology in vitro : an international journal published in association with BIBRA · 2022Article
- Sortilin restricts secretion of apolipoprotein B-100 by hepatocytes under stressed but not basal conditions.The Journal of clinical investigation · 2022Article
- Identification of Sortilin Alternatively Spliced Variants in Mouse 3T3L1 Adipocytes.International journal of molecular sciences · 2021Article
- An Allosteric Binding Site on Sortilin Regulates the Trafficking of VLDL, PCSK9, and LDLR in Hepatocytes.Biochemistry · 2020Article
- Sortilin levels correlate with major cardiovascular events of diabetic patients with peripheral artery disease following revascularization: a prospective study.Cardiovascular diabetology · 2020Article
- Sortilin levels are associated with peripheral arterial disease in type 2 diabetic subjects.Cardiovascular diabetology · 2019Article
- "Trans-nonachlor increases extracellular free fatty acid accumulation and de novo lipogenesis to produce hepatic steatosis in McArdle-RH7777 cells".Toxicology in vitro : an international journal published in association with BIBRA · 2018Article
- Autophagy Is Required for Sortilin-Mediated Degradation of Apolipoprotein B100.Circulation research · 2018Article
- Hepatic ABCA1 deficiency is associated with delayed apolipoprotein B secretory trafficking and augmented VLDL triglyceride secretion.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2017Article
- Complex role of autophagy in regulation of hepatic lipid and lipoprotein metabolism.Journal of biomedical research · 2017Article
- Phosphatidylinositol (3,4,5)-trisphosphate binds to sortilin and competes with neurotensin: Implications for very low density lipoprotein binding.Biochemical and biophysical research communications · 2016Article
- Sortilin facilitates VLDL-B100 secretion by insulin sensitive McArdle RH7777 cells.Biochemical and biophysical research communications · 2016Article
- Insulin dependent apolipoprotein B degradation and phosphatidylinositide 3-kinase activation with microsomal translocation are restored in McArdle RH7777 cells following serum deprivation.Biochemical and biophysical research communications · 2016Article
- The enigmatic role of sortilin in lipoprotein metabolism.Current opinion in lipidology · 2015Article
- Insulin resistance induces posttranslational hepatic sortilin 1 degradation in mice.The Journal of biological chemistry · 2015Article
- Sortilin and lipoprotein metabolism: making sense out of complexity.Current opinion in lipidology · 2014Review
- Apolipoprotein B100 quality control and the regulation of hepatic very low density lipoprotein secretion.Journal of biomedical research · 2014Article
- Lipoprotein metabolism, dyslipidemia, and nonalcoholic fatty liver disease.Seminars in liver disease · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Insulin suppresses secretion of very low density lipoprotein (VLDL) apolipoprotein (apo) B in primary rodent hepatocytes (RH) by favoring the degradation of B100, the larger form of apo B, through post-endoplasmic reticulum proteolysis. Sortilin 1 (sort1), a multi-ligand sorting receptor, has been proposed as a mediator of lysosomal B100 degradation by directing B100 in pre-VLDL to lysosomes rather than allowing maturation to VLDL and secretion. The purpose of our studies was to investigate the role of sort1 in insulin-dependent degradation of apo B. Using liver derived McArdle RH7777 (McA) cells, we demonstrate that insulin suppresses VLDL B100 secretion via a phosphatidylinositide 3-kinase (PI3K) dependent process that is inhibitable by wortmannin in a fashion similar to RH. Using McA cells and in situ cross-linking, we demonstrate that insulin acutely (30min) stimulates the interaction of B100 with sort1. The insulin-induced interaction of sort1-B100 is markedly enhanced when lysosomal degradation is inhibited by Bafilomycin A1 (BafA1), an inhibitor of lysosomal acidification. As BafA1 also prevents insulin suppressive effects on apo B secretion, our results suggest that sort1-B100 interaction stimulated by insulin transiently accumulates with BafA1 and favors B100 secretion by default.
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