ArticleEukaryotic cell2013
Role of Cryptococcus neoformans Rho1 GTPases in the PKC1 signaling pathway in response to thermal stress.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 38 citations in OpenAlex.
- Reactive Oxygen Species-Responsive Signaling Networks and Oxidative Stress Adaptation in Critical Priority Fungal Pathogens.Journal of fungi (Basel, Switzerland) · 2026Review
- Conserved and rewired MAPK networks in human fungal pathogens.FEMS yeast research · 2026Review
- Kinome analysis of Madurella mycetomatis identified kinases in the cell wall integrity pathway as novel potential therapeutic drug targets in eumycetoma caused by Madurella mycetomatis.PLoS neglected tropical diseases · 2025Article
- Impact of Lysine Succinylation on the Biology of Fungi.Current issues in molecular biology · 2024Review
- Cryptococcus neoformans requires the TVF1 gene for thermotolerance and virulence.Medical mycology · 2023Article
- Developing novel antifungals: lessons from G protein-coupled receptors.Trends in pharmacological sciences · 2023Review
- Article
- Epistatic genetic interactions govern morphogenesis during sexual reproduction and infection in a global human fungal pathogen.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The monothiol glutaredoxin Grx4 influences thermotolerance, cell wall integrity, and Mpk1 signaling in Cryptococcus neoformans.G3 (Bethesda, Md.) · 2021Article
- Cell Wall Integrity Pathway Involved in Morphogenesis, Virulence and Antifungal Susceptibility inJournal of fungi (Basel, Switzerland) · 2021Review
- A Unique Dual-Readout High-Throughput Screening Assay To Identify Antifungal Compounds with Aspergillus fumigatus.mSphere · 2021Article
- Protein Interactions of the Mechanosensory Proteins Wsc2 and Wsc3 for Stress Resistance inG3 (Bethesda, Md.) · 2020Article
- Environmental Factors That Contribute to the Maintenance ofMicroorganisms · 2020Review
- Landscape of gene expression variation of natural isolates ofMicrobial genomics · 2020Article
- A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome.Fungal genetics and biology : FG & B · 2017Article
- The first succinylome profile of Trichophyton rubrum reveals lysine succinylation on proteins involved in various key cellular processes.BMC genomics · 2017Article
- The role of Aspartyl aminopeptidase (Ape4) in Cryptococcus neoformans virulence and authophagy.PloS one · 2017Article
- A Single Protein S-acyl Transferase Acts through Diverse Substrates to Determine Cryptococcal Morphology, Stress Tolerance, and Pathogenic Outcome.PLoS pathogens · 2015Article
- The role of the de novo pyrimidine biosynthetic pathway in Cryptococcus neoformans high temperature growth and virulence.Fungal genetics and biology : FG & B · 2014Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
To initiate and establish infection in mammals, the opportunistic fungal pathogen Cryptococcus neoformans must survive and thrive upon subjection to host temperature. Primary maintenance of cell integrity is controlled through the protein kinase C1 (PKC1) signaling pathway, which is regulated by a Rho1 GTPase in Saccharomyces cerevisiae. We identified three C. neoformans Rho GTPases, Rho1, Rho10, and Rho11, and have begun to elucidate their role in growth and activation of the PKC1 pathway in response to thermal stress. Western blot analysis revealed that heat shock of wild-type cells resulted in phosphorylation of Mpk1 mitogen-activated protein kinase (MAPK). Constitutive activation of Rho1 caused phosphorylation of Mpk1 independent of temperature, indicating its role in pathway regulation. A strain with a deletion of RHO10 also displayed this constitutive Mpk1 phosphorylation phenotype, while one with a deletion of RHO11 yielded phosphorylation similar to that of wild type. Surprisingly, like a rho10Δ strain, a strain with a deletion of both RHO10 and RHO11 displayed temperature sensitivity but mimicked wild-type phosphorylation, which suggests that Rho10 and Rho11 have coordinately regulated functions. Heat shock-induced Mpk1 phosphorylation also required the PKC1 pathway kinases Bck1 and Mkk2. However, Pkc1, thought to be the major regulatory kinase of the cell integrity pathway, was dispensable for this response. Together, our results argue that Rho proteins likely interact via downstream components of the PKC1 pathway or by alternative pathways to activate the cell integrity pathway in C. neoformans.
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