ArticleVascular cell2012
Regulation of endothelial permeability and transendothelial migration of cancer cells by tropomyosin-1 phosphorylation.
Article in Vascular cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
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- Angiopoietin-like protein 2 mediates vasculopathy-driven fibrogenesis in a mouse model of systemic sclerosis.The Journal of clinical investigation · 2025Article
- Uncovering endothelial to mesenchymal transition drivers in atherosclerosis via multi-omics analysis.BMC cardiovascular disorders · 2025Article
- Interaction of Drug-Sensitive and -Resistant Human Melanoma Cells with HUVEC Cells: A Label-Free Cell-Based Impedance Study.Biomedicines · 2023Article
- Emerging Role of Plant-Based Dietary Components in Post-Translational Modifications Associated with Colorectal Cancer.Life (Basel, Switzerland) · 2023Review
- Identification of hub genes and regulatory networks in histologically unstable carotid atherosclerotic plaque by bioinformatics analysis.BMC medical genomics · 2022Article
- A reciprocal feedback of miR-548ac/YB-1/Snail induces EndMT of HUVECs during acidity microenvironment.Cancer cell international · 2021Article
- HA and HS Changes in Endothelial Inflammatory Activation.Biomolecules · 2021Article
- Endothelial cells and organ function: applications and implications of understanding unique and reciprocal remodelling.The FEBS journal · 2020Review
- 6-Gingerol stabilized the p-VEGFR2/VE-cadherin/β-catenin/actin complex promotes microvessel normalization and suppresses tumor progression.Journal of experimental & clinical cancer research : CR · 2019Article
- Biofabrication strategies for creating microvascular complexity.Biofabrication · 2019Review
- Stress phase angle regulates differentiation of human adipose-derived stem cells toward endothelial phenotype.Progress in biomaterials · 2018Article
- Fluorescence tumor imaging by i.v. administered indocyanine green in a mouse model of colitis-associated colon cancer.Cancer science · 2018Article
- Proteomic analysis and comparison of intra‑ and extracranial cerebral atherosclerosis responses to hyperlipidemia in rabbits.Molecular medicine reports · 2017Article
- The p38 pathway, a major pleiotropic cascade that transduces stress and metastatic signals in endothelial cells.Oncotarget · 2017Review
- Visualization of endothelial barrier damage prior to formation of atherosclerotic plaques.Histochemistry and cell biology · 2017Article
- Angiopoietin-like 4: A double-edged sword in atherosclerosis and ischemic stroke?Experimental neurology · 2015Article
- Carbonic anhydrase activation is associated with worsened pathological remodeling in human ischemic diabetic cardiomyopathy.Journal of the American Heart Association · 2014Article
- Recombinant Treponema pallidum protein Tp0965 activates endothelial cells and increases the permeability of endothelial cell monolayer.PloS one · 2014Article
- Structure and biomechanics of the endothelial transcellular circumferential invasion array in tumor invasion.PloS one · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
unlabelled
backgroundLoss of endothelial cell integrity and selective permeability barrier is an early event in the sequence of oxidant-mediated injury and may result in atherosclerosis, hypertension and facilitation of transendothelial migration of cancer cells during metastasis. We already reported that endothelial cell integrity is tightly regulated by the balanced co-activation of p38 and ERK pathways. In particular, we showed that phosphorylation of tropomyosin-1 (tropomyosin alpha-1 chain = Tm1) at Ser283 by DAP kinase, downstream of the ERK pathway might be a key event required to maintain the integrity and normal functions of the endothelium in response to oxidative stress.
methodsEndothelial permeability was assayed by monitoring the passage of Dextran-FITC through a tight monolayer of HUVECs grown to confluence in Boyden chambers. Actin and Tm1 dynamics and distribution were evaluated by immunofluorescence. We modulated the expression of Tm1 by siRNA and lentiviral-mediated expression of wild type and mutated forms of Tm1 insensitive to the siRNA. Transendothelial migration of HT-29 colon cancer cells was monitored in Boyden chambers similarly as for permeability.
resultsWe provide evidence indicating that Tm1 phosphorylation at Ser283 is essential to regulate endothelial permeability under oxidative stress by modulating actin dynamics. Moreover, the transendothelial migration of colon cancer cells is also regulated by the phosphorylation of Tm1 at Ser283.
conclusionOur finding strongly support the role for the phosphorylation of endothelial Tm1 at Ser283 to prevent endothelial barrier dysfunction associated with oxidative stress injury.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.