ArticleBMC microbiology2012
PHB granules are attached to the nucleoid via PhaM in Ralstonia eutropha.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 85 citations in OpenAlex.
- Bacteria producing the bioplastic polyhydroxybutyrate kill the nematode Caenorhabditis elegans.PLoS biology · 2026Article
- Gene expression analysis reveals distinct PHB depolymerization mechanisms and broader involvement of the PHB cycle in Rhodospirillum rubrum growing on acetate and fructose.Microbial cell factories · 2026Article
- Phasins PhbP2 and PhbP3 Are Involved in the Depolymerization of Polyhydroxybutyrate in Azotobacter vinelandii.Journal of basic microbiology · 2025Article
- Organizing organelles: bacterial strategies for localizing intracellular compartments.Current opinion in microbiology · 2025Review
- Polyphosphate: The "Dark Matter" of Bacterial Chromatin Structure.Molecular microbiology · 2025Review
- Cultivation driven transcriptomic changes in the wild-type and mutant strains ofComputational and structural biotechnology journal · 2024Article
- Quantitative Analysis ofBiomolecules · 2024Article
- Unveiling the repressive mechanism of a PPS-like regulator (PspR) in polyhydroxyalkanoates biosynthesis network.Applied microbiology and biotechnology · 2024Article
- Computational Analysis of the Tripartite Interaction of Phasins (PhaP4 and 5)-Sigma Factor (σPolymers · 2024Article
- McaA and McaB control the dynamic positioning of a bacterial magnetic organelle.Nature communications · 2022Article
- Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology.Microorganisms · 2022Article
- Heterologous phasin expression inMetabolic engineering communications · 2022Article
- Lab-Scale Cultivation ofBioengineering (Basel, Switzerland) · 2022Article
- An updated overview on the regulatory circuits of polyhydroxyalkanoates synthesis.Microbial biotechnology · 2022Review
- The role of NAD and NAD precursors on longevity and lifespan modulation in the budding yeast, Saccharomyces cerevisiae.Biogerontology · 2022Review
- Three-dimensional label-free visualization and quantification of polyhydroxyalkanoates in individual bacterial cell in its native state.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- 3-Hydroxybutyrate as a Metabolite and a Signal Molecule Regulating Processes of Living Organisms.Biomolecules · 2021Review
- No endospore formation confirmed in members of the phylum Proteobacteria.Applied and environmental microbiology · 2021Article
- A Compass To Boost Navigation: Cell Biology of Bacterial Magnetotaxis.Journal of bacteriology · 2020Review
- Phasin PhaP1 is involved in polyhydroxybutyrate granules morphology and in controlling early biopolymer accumulation in Azospirillum brasilense Sp7.AMB Express · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPoly(3-hydroxybutyrate) (PHB) granules are important storage compounds of carbon and energy in many prokaryotes which allow survival of the cells in the absence of suitable carbon sources. Formation and subcellular localization of PHB granules was previously assumed to occur randomly in the cytoplasm of PHB accumulating bacteria. However, contradictionary results on subcellular localization of PHB granules in Ralstonia eutropha were published, recently.
resultsHere, we provide evidence by transmission electron microscopy that PHB granules are localized in close contact to the nucleoid region in R. eutropha during growth on nutrient broth. Binding of PHB granules to the nucleoid is mediated by PhaM, a PHB granule associated protein with phasin-like properties that is also able to bind to DNA and to phasin PhaP5. Over-expression of PhaM resulted in formation of many small PHB granules that were always attached to the nucleoid region. In contrast, PHB granules of ∆phaM strains became very large and distribution of granules to daughter cells was impaired. Association of PHB granules to the nucleoid region was prevented by over-expression of PhaP5 and clusters of several PHB granules were mainly localized near the cell poles.
conclusionSubcellular localization of PHB granules is controlled in R. eutropha and depends on the presence and concentrations of at least two PHB granule associated proteins, PhaM and PhaP5.
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