ArticleTissue engineering. Part A2013
Development of a three-dimensional bone-like construct in a soft self-assembling peptide matrix.
Article in Tissue engineering. Part A, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- 3D coaxial bioprinting of RADA16-I self-assembling peptide hydrogel.Materials today. Bio · 2026Article
- Self-assembling peptide nanofibers as growth factor-mimicking scaffolds enhancing the bone regeneration potential of nanoceramics: a triad ofRSC advances · 2025Article
- Treatment of large bone defects in load-bearing bone: traditional and novel bone grafts.Journal of Zhejiang University. Science. B · 2025Review
- Unveiling the superior function of RADA in bone regeneration compared to KSL as two critical cores within self-assembling peptide nanofibers: Insights from in vitro and in vivo studies.Regenerative therapy · 2024Article
- β-Sheet to Random Coil Transition in Self-Assembling Peptide Scaffolds Promotes Proteolytic Degradation.Biomolecules · 2022Article
- Erlotinib Promotes Ligand-Induced EGFR Degradation in 3D but Not 2D Cultures of Pancreatic Ductal Adenocarcinoma Cells.Cancers · 2021Article
- Natural Polymeric Scaffolds in Bone Regeneration.Frontiers in bioengineering and biotechnology · 2020Review
- Development of a Three-Dimensional Bioengineered Platform for Articular Cartilage Regeneration.Biomolecules · 2019Article
- Stimuli-Responsive Supramolecular Hydrogels and Their Applications in Regenerative Medicine.Macromolecular bioscience · 2019Review
- Dedifferentiated Human Articular Chondrocytes Redifferentiate to a Cartilage-Like Tissue Phenotype in a Poly(ε-Caprolactone)/Self-Assembling Peptide Composite Scaffold.Materials (Basel, Switzerland) · 2016Article
- Chondroitin Sulfate- and Decorin-Based Self-Assembling Scaffolds for Cartilage Tissue Engineering.PloS one · 2016Article
- A facile in vitro model to study rapid mineralization in bone tissues.Biomedical engineering online · 2014Article
- Nanobiotechnology and bone regeneration: a mini-review.International orthopaedics · 2014Review
- Scaffold design for bone regeneration.Journal of nanoscience and nanotechnology · 2014Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This work describes the development of a three-dimensional (3D) model of osteogenesis using mouse preosteoblastic MC3T3-E1 cells and a soft synthetic matrix made out of self-assembling peptide nanofibers. By adjusting the matrix stiffness to very low values (around 120 Pa), cells were found to migrate within the matrix, interact forming a cell-cell network, and create a contracted and stiffer structure. Interestingly, during this process, cells spontaneously upregulate the expression of bone-related proteins such as collagen type I, bone sialoprotein, and osteocalcin, indicating that the 3D environment enhances their osteogenic potential. However, unlike MC3T3-E1 cultures in 2D, the addition of dexamethasone is required to acquire a final mature phenotype characterized by features such as matrix mineralization. Moreover, a slight increase in the hydrogel stiffness (threefold) or the addition of a cell contractility inhibitor (Rho kinase inhibitor) abrogates cell elongation, migration, and 3D culture contraction. However, this mechanical inhibition does not seem to noticeably affect the osteogenic process, at least at early culture times. This 3D bone model intends to emphasize cell-cell interactions, which have a critical role during tissue formation, by using a compliant unrestricted synthetic matrix.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.