ArticleJournal of the American Chemical Society2013
Multiplexed protease activity assay for low-volume clinical samples using droplet-based microfluidics and its application to endometriosis.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 89 citations in OpenAlex.
- Exploring endometriosis through 3DMaterials today. Bio · 2026Review
- Evaluation of single-cell heterogeneity and invasive potential in cancer cells via secreted protease activity assay.Analytica chimica acta · 2025Article
- From specialization to broad adoption: Key trends in droplet microfluidic innovations enhancing accessibility to non-experts.Biomicrofluidics · 2025Article
- Organ-on-a-chip: future of female reproductive pathophysiological models.Journal of nanobiotechnology · 2024Review
- Bioengineering approaches for the endometrial research and application.Materials today. Bio · 2024Review
- Simple and Rapid Method of Microwell Array Fabrication for Drug Testing on 3D Cancer Spheroids.ACS omega · 2024Article
- Revolutionizing the female reproductive system research using microfluidic chip platform.Journal of nanobiotechnology · 2023Review
- Profiling of Multiple Matrix Metalloproteinases Activities in the Progression of Osteosarcoma by Peptide Microarray-Based Fluorescence Assay on Polymer Brush-Coated Zinc Oxide Nanorod Substrate.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Droplet Detection and Sorting System in Microfluidics: A Review.Micromachines · 2022Review
- Strategies for modelling endometrial diseases.Nature reviews. Endocrinology · 2022Review
- Rapid parallel generation of a fluorescently barcoded drop library from a microtiter plate using the plate-interfacing parallel encapsulation (PIPE) chip.Lab on a chip · 2022Article
- The Tumor Proteolytic Landscape: A Challenging Frontier in Cancer Diagnosis and Therapy.International journal of molecular sciences · 2021Review
- Activatable Zymography Probes EnableCancer research · 2021Article
- Bioengineering models of female reproduction.Bio-design and manufacturing · 2020Article
- Activity-Based Diagnostics: An Emerging Paradigm for Disease Detection and Monitoring.Trends in molecular medicine · 2020Review
- Sequentially addressable dielectrophoretic array for high-throughput sorting of large-volume biological compartments.Science advances · 2020Article
- Luminescent nanomaterials for droplet tracking in a microfluidic trapping array.Analytical and bioanalytical chemistry · 2019Article
- Customizing droplet contents and dynamic ranges via integrated programmable picodroplet assembler.Microsystems & nanoengineering · 2019Article
- Droplet microfluidics for high-sensitivity and high-throughput detection and screening of disease biomarkers.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Functional profiling of circulating tumor cells with an integrated vortex capture and single-cell protease activity assay.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
As principal degrading enzymes of the extracellular matrix, metalloproteinases (MPs) contribute to various pathologies and represent a family of promising drug targets and biomarker candidates. However, multiple proteases and endogenous inhibitors interact to govern MP activity, often leading to highly context-dependent protease function that unfortunately has impeded associated clinical utility. We present a method for rapidly assessing the activity of multiple specific proteases in small volumes (<20 μL) of complex biological fluids such as clinical samples that are available only in very limited amounts. It uses a droplet-based microfluidic platform that injects the sample into thousands of picoliter-scale droplets from a barcoded droplet library (DL) containing mixtures of unique, moderately selective FRET-based protease substrates and specific inhibitors and monitors hundreds of the reactions thus initiated simultaneously by tracking these droplets. Specific protease activities in the sample are then inferred from the reaction rates using a deconvolution technique, proteolytic activity matrix analysis (PrAMA). Using a nine-member DL with three inhibitors and four FRET substrates, we applied the method to the peritoneal fluid of subjects with and without the invasive disease endometriosis. The results showed clear and physiologically relevant differences with disease, in particular, decreased MMP-2 and ADAM-9 activities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.