ArticleJournal of the American Chemical Society2012
Tetarimycin A, an MRSA-active antibiotic identified through induced expression of environmental DNA gene clusters.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed, 95 citations in OpenAlex.
- Karakum desert: a unique source of cultivable novel and rare actinomycetes with a remarkable biosynthetic potential.World journal of microbiology & biotechnology · 2025Article
- Diversity and potential functional role of phyllosphere-associated actinomycetota isolated from cupuassu (Theobroma grandiflorum) leaves: implications for ecosystem dynamics and plant defense strategies.Molecular genetics and genomics : MGG · 2024Article
- An unusual aromatase/cyclase programs the formation of the phenyldimethylanthrone framework in anthrabenzoxocinones and fasamycin.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Mechanisms of Antibiotic Resistance and Developments in Therapeutic Strategies to CombatInfection and drug resistance · 2024Review
- Mining metagenomes reveals diverse antibiotic biosynthetic genes in uncultured microbial communities.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- A Convenient Diels-Alder Approach toward Potential Polyketide-like Antibiotics Using α-Activated α,β-Unsaturated 4,4-Dimethyl-1-tetralones as Dienophiles.Molecules (Basel, Switzerland) · 2023Article
- Fighting antibiotic resistance-strategies and (pre)clinical developments to find new antibacterials.EMBO reports · 2023Review
- Identification of PKS Gene Clusters from Metagenomic Libraries Using a Next-Generation Sequencing Approach.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Regulated Expression of an Environmental DNA-Derived Type II Polyketide Gene Cluster in Streptomyces Hosts Identified a New Tetracenomycin Derivative TCM Y.Current microbiology · 2022Article
- Nocardia noduli sp. nov., a novel actinobacterium with biotechnological potential.Archives of microbiology · 2022Article
- Genome insights into the pharmaceutical and plant growth promoting features of the novel species Nocardia alni sp. nov.BMC genomics · 2022Article
- Combined assembly of long and short sequencing reads improve the efficiency of exploring the soil metagenome.BMC genomics · 2022Article
- Review
- Discovery of Novel Biosynthetic Gene Cluster Diversity From a Soil Metagenomic Library.Frontiers in microbiology · 2020Article
- Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering.Microbial biotechnology · 2019Review
- Heterologous expression-facilitated natural products' discovery in actinomycetes.Journal of industrial microbiology & biotechnology · 2019Review
- Atolypenes, Tricyclic Bacterial Sesterterpenes Discovered Using a Multiplexed In Vitro Cas9-TAR Gene Cluster Refactoring Approach.ACS synthetic biology · 2019Article
- Response of Secondary Metabolism of Hypogean Actinobacterial Genera to Chemical and Biological Stimuli.Applied and environmental microbiology · 2018Article
- Recent advances in activating silent biosynthetic gene clusters in bacteria.Current opinion in microbiology · 2018Review
- Determination of bacterial DNA based on catalytic oxidation of cysteine by G-quadruplex DNAzyme generated from asymmetric PCR: Application to the colorimetric detection of Staphylococcus aureus.Mikrochimica acta · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The propagation of DNA extracted directly from environmental samples in laboratory-grown bacteria provides a means to study natural products encoded in the genomes of uncultured bacteria. However, gene silencing often hampers the functional characterization of gene clusters captured on environmental DNA clones. Here we show that the overexpression of transcription factors found in sequenced environmental DNA-derived biosynthetic gene clusters, in conjunction with traditional culture-broth extract screening, can be used to identify new bioactive secondary metabolites from otherwise-silent gene clusters. Tetarimycin A, a tetracyclic methicillin-resistant Staphylococcus aureus (MRSA)-active antibiotic, was isolated from the culture-broth extract of Streptomyces albus cultures cotransformed with an environmentally derived type-II polyketide biosynthetic gene cluster and its pathway-specific Streptomyces antibiotic regulatory protein (SARP) cloned under the control of the constitutive ermE* promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.