ArticlePloS one2012
Probing mixed-genotype infections I: extraction and cloning of infections from hosts of the trypanosomatid Crithidia bombi.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 78 citations in OpenAlex.
- Role of ergosterol biosynthesis in growth, drug sensitivity, and host colonization of honey bee trypanosomatid parasite,FEMS microbes · 2026Article
- Culture and Genetic Modification of the Monoxenous Trypanosomatids Crithidia fasciculata and Crithidia bombi.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Chromosome-level genome assembly of trypanosomatid parasite Lotmaria passim links chromosome duplication and divergence with infection of honey bees.BMC genomics · 2025Article
- BBSome deficiency in Lotmaria passim reveals divergent functions in trypanosomatid parasites.Parasites & vectors · 2025Article
- Somy evolution in the honey bee infecting trypanosomatid parasite Lotmaria passim.G3 (Bethesda, Md.) · 2025Article
- Protein subcellular relocalization and function of duplicated flagellar calcium binding protein genes in honey bee trypanosomatid parasite.PLoS genetics · 2024Article
- Aspartyl protease in the secretome of honey bee trypanosomatid parasite contributes to infection of bees.Parasites & vectors · 2024Article
- A simulated natural heatwave perturbs bumblebee immunity and resistance to infection.The Journal of animal ecology · 2024Article
- High temperatures augment inhibition of parasites by a honey bee gut symbiont.Applied and environmental microbiology · 2023Article
- Host and gut microbiome modulate the antiparasitic activity of nectar metabolites in a bumblebee pollinator.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
- Genetic variation and microbiota in bumble bees cross-infected by different strains of C. bombi.PloS one · 2022Article
- Hot and sour: parasite adaptations to honeybee body temperature and pH.Proceedings. Biological sciences · 2021Article
- Workflow ofInternational journal for parasitology. Parasites and wildlife · 2021Article
- Cross-infectivity of honey and bumble bee-associated parasites across three bee families.Parasitology · 2020Article
- Trypanosomatid parasite dynamically changes the transcriptome during infection and modifies honey bee physiology.Communications biology · 2020Article
- Temperature dependence of parasitic infection and gut bacterial communities in bumble bees.Environmental microbiology · 2019Article
- Infection Outcomes are Robust to Thermal Variability in a Bumble Bee Host-Parasite System.Integrative and comparative biology · 2019Article
- Genomic Variation among Strains ofmSphere · 2019Article
- pH-mediated inhibition of a bumble bee parasite by an intestinal symbiont.Parasitology · 2019Article
- Gene Disruption of Honey Bee Trypanosomatid Parasite,Frontiers in cellular and infection microbiology · 2019Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
We here present an efficient, precise and reliable method to isolate and cultivate healthy and viable single Crithidia bombi cells from bumblebee faeces using flow cytometry. We report a precision of >99% in obtaining single trypanosomatid cells for further culture and analysis ("cloning"). In the study, we have investigated the use of different liquid media to cultivate C. bombi and present an optimal medium for obtaining viable clones from all tested, infected host donors. We show that this method can be applied to genotype a collection of clones from natural infections. Furthermore, we show how to cryo-preserve C. bombi cells to be revived to become infective clones after at least 4 years of storage. Considering the high prevalence of infections in natural populations, our method provides a powerful tool in studying the level and diversity of these infections, and thus enriches the current methodology for the studies of complex host-parasite interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.