ArticlePloS one2012
Barley HvHMA1 is a heavy metal pump involved in mobilizing organellar Zn and Cu and plays a role in metal loading into grains.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 99 citations in OpenAlex.
- Genome-wide analysis ofFrontiers in plant science · 2025Article
- Molecular Mechanisms of Plant Responses to Copper: From Deficiency to Excess.International journal of molecular sciences · 2024Review
- Cadmium Stress Signaling Pathways in Plants: Molecular Responses and Mechanisms.Current issues in molecular biology · 2024Review
- A two-sequence motif-based method for the inventory of gene families in fragmented and poorly annotated genome sequences.BMC genomics · 2024Article
- Mapping QTL for Mineral Accumulation and Shoot Dry Biomass in Barley under Different Levels of Zinc Supply.International journal of molecular sciences · 2023Article
- Deciphering the functional roles of transporter proteins in subcellular metal transportation of plants.Planta · 2023Review
- Article
- Physiological and Molecular Mechanisms of Plant Responses to Copper Stress.International journal of molecular sciences · 2022Review
- Zinc Transporter ZmLAZ1-4 Modulates Zinc Homeostasis on Plasma and Vacuolar Membrane in Maize.Frontiers in plant science · 2022Article
- The Mechanism of Metal Homeostasis in Plants: A New View on the Synergistic Regulation Pathway of Membrane Proteins, Lipids and Metal Ions.Membranes · 2021Review
- Genome-Wide Identification of thePlants (Basel, Switzerland) · 2021Article
- A thorough screening based on QTLs controlling zinc and copper accumulation in the grain of different wheat genotypes.Environmental science and pollution research international · 2021Article
- Transcriptome Response to Cadmium Exposure in Barley (Frontiers in plant science · 2021Article
- Copper excess reduces nitrate uptake by Arabidopsis roots with specific effects on gene expression.Journal of plant physiology · 2018Article
- Article
- OsMTP11 is localised at the Golgi and contributes to Mn tolerance.Scientific reports · 2017Article
- F-group bZIPs in barley-a role in Zn deficiency.Plant, cell & environment · 2017Article
- Functional and Biochemical Characterization of Cucumber Genes Encoding Two Copper ATPases CsHMA5.1 and CsHMA5.2.The Journal of biological chemistry · 2015Article
- Regulation of Zn and Fe transporters by the GPC1 gene during early wheat monocarpic senescence.BMC plant biology · 2014Article
- Gli1 contributes to cellular resistance to cisplatin through altered cellular accumulation of the drug.Oncology reports · 2014Article
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heavy metal transporters belonging to the P(1B)-ATPase subfamily of P-type ATPases are key players in cellular heavy metal homeostasis. Heavy metal transporters belonging to the P(1B)-ATPase subfamily of P-type ATPases are key players in cellular heavy metal homeostasis. In this study we investigated the properties of HvHMA1, which is a barley orthologue of Arabidopsis thaliana AtHMA1 localized to the chloroplast envelope. HvHMA1 was localized to the periphery of chloroplast of leaves and in intracellular compartments of grain aleurone cells. HvHMA1 expression was significantly higher in grains compared to leaves. In leaves, HvHMA1 expression was moderately induced by Zn deficiency, but reduced by toxic levels of Zn, Cu and Cd. Isolated barley chloroplasts exported Zn and Cu when supplied with Mg-ATP and this transport was inhibited by the AtHMA1 inhibitor thapsigargin. Down-regulation of HvHMA1 by RNA interference did not have an effect on foliar Zn and Cu contents but resulted in a significant increase in grain Zn and Cu content. Heterologous expression of HvHMA1 in heavy metal-sensitive yeast strains increased their sensitivity to Zn, but also to Cu, Co, Cd, Ca, Mn, and Fe. Based on these results, we suggest that HvHMA1 is a broad-specificity exporter of metals from chloroplasts and serve as a scavenging mechanism for mobilizing plastid Zn and Cu when cells become deficient in these elements. In grains, HvHMA1 might be involved in mobilizing Zn and Cu from the aleurone cells during grain filling and germination.
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