ArticlePloS one2012
Isolation and characterization of human dental pulp derived stem cells by using media containing low human serum percentage as clinical grade substitutes for bovine serum.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Validated methods for isolation and qualification of mesenchymal stromal/stem cells from different sources.Journal of translational medicine · 2025Review
- Good manufacturing practice production of human corneal limbus-derived stromal stem cells and in vitro quality screening for therapeutic inhibition of corneal scarring.Stem cell research & therapy · 2024Article
- Minor salivary gland stem cells: a comparative study of the biological properties under clinical-grade culture conditions.Cell and tissue research · 2023Article
- Article
- Study of optimal conditions for growth and osteogenic differentiation of dental pulp stem cells based on glucose and serum content.Saudi journal of biological sciences · 2021Article
- Search for Novel Plasma Membrane Proteins as Potential Biomarkers in Human Mesenchymal Stem Cells Derived from Dental Pulp, Adipose Tissue, Bone Marrow, and Hair Follicle.The Journal of membrane biology · 2021Article
- Similar Features, Different Behaviors: A Comparative In VitroStudy of the Adipogenic Potential of Stem Cells from Human Follicle, Dental Pulp, and Periodontal Ligament.Journal of personalized medicine · 2021Article
- Effect of Ascorbic Acid on Differentiation, Secretome and Stemness of Stem Cells from Human Exfoliated Deciduous Tooth (SHEDs).Journal of personalized medicine · 2021Article
- Dental Pulp Stem Cells Derived From Adult Human Third Molar Tooth: A Brief Review.Frontiers in cell and developmental biology · 2021Review
- Chemically Defined Conditions Mediate an Efficient Induction of Dental Pulp Pluripotent-Like Stem Cells into Hepatocyte-Like Cells.Stem cells international · 2021Article
- Comparison of the Migration Potential through Microperforated Membranes of CD146+ GMSC Population versus Heterogeneous GMSC Population.Stem cells international · 2021Article
- Establishment and neural differentiation of neural crest-derived stem cells from human dental pulp in serum-free conditions.Stem cells translational medicine · 2020Article
- Regenerative Capacity of Dental Pulp Stem Cells: A Systematic Review.Journal of pharmacy & bioallied sciences · 2020Review
- 2,4-D causes oxidative stress induction and apoptosis in human dental pulp stem cells (hDPSCs).Environmental science and pollution research international · 2019Article
- Aspirin promotes osteogenic differentiation of human dental pulp stem cells.International journal of molecular medicine · 2018Article
- Directed reprogramming of comprehensively characterized dental pulp stem cells extracted from natal tooth.Scientific reports · 2018Article
- Isolation and prolonged expansion of oral mesenchymal stem cells under clinical-grade, GMP-compliant conditions differentially affects "stemness" properties.Stem cell research & therapy · 2017Article
- Dental Pulp Stem Cells: Current Advances in Isolation, Expansion and Preservation.Tissue engineering and regenerative medicine · 2017Review
- Human DPSCs fabricate vascularized woven bone tissue: a new tool in bone tissue engineering.Clinical science (London, England : 1979) · 2017Article
- Stem Cells from Dental Pulp: What Epigenetics Can Do with Your Tooth.Frontiers in physiology · 2017Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adult stem cells have been proposed as an alternative to embryonic stem cells to study multilineage differentiation in vitro and to use in therapy. Current culture media for isolation and expansion of adult stem cells require the use of large amounts of animal sera, but animal-derived culture reagents give rise to some questions due to the real possibility of infections and severe immune reactions. For these reasons a clinical grade substitute to animal sera is needed. We tested the isolation, proliferation, morphology, stemness related marker expression, and osteoblastic differentiation potential of Dental Pulp Stem Cells (DPSC) in a chemically defined medium containing a low percentage of human serum, 1.25%, in comparison to a medium containing 10% Fetal Bovine Serum (FBS). DPSCs cultured in presence of our isolation/proliferation medium added with low HS percentage were obtained without immune-selection methods and showed high uniformity in the expression of stem cell markers, proliferated at higher rate, and demonstrated comparable osteoblastic potential with respect to DPSCs cultured in 10% FBS. In this study we demonstrated that a chemically defined medium added with low HS percentage, derived from autologous and heterologous sources, could be a valid substitute to FBS-containing media and should be helpful for adult stem cells clinical application.
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Registered trials
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