ArticlePloS one2012
Cell-type specific expression of the vasopressin gene analyzed by AAV mediated gene delivery of promoter deletion constructs into the rat SON in vivo.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- FOXP1 is differentially active during development of murine vasopressin and oxytocin magnocellular neurons.iScience · 2026Article
- Optimization of AAV vectors for transactivator-regulated enhanced gene expression within targeted neuronal populations.iScience · 2024Article
- Article
- Efficiency of cell-type specific and generic promoters in transducing oxytocin neurons and monitoring their neural activity during lactation.Scientific reports · 2021Article
- Effects of salt-loading on supraoptic vasopressin neurones assessed by ClopHensorN chloride imaging.Journal of neuroendocrinology · 2019Article
- Viral rescue of magnocellular vasopressin cells in adolescent Brattleboro rats ameliorates diabetes insipidus, but not the hypoaroused phenotype.Scientific reports · 2019Article
- Strategies for targeting primate neural circuits with viral vectors.Journal of neurophysiology · 2016Review
- A RNA-Seq Analysis of the Rat Supraoptic Nucleus Transcriptome: Effects of Salt Loading on Gene Expression.PloS one · 2015Article
- Transcription factor CREB3L1 regulates vasopressin gene expression in the rat hypothalamus.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- MicroRNA profiling in the mouse hypothalamus reveals oxytocin-regulating microRNA.Journal of neurochemistry · 2013Article
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
The magnocellular neurons (MCNs) in the supraoptic nucleus (SON) of the hypothalamus selectively express either oxytocin (Oxt) or vasopressin (Avp) neuropeptide genes. In this paper we examine the cis-regulatory domains in the Avp gene promoter that are responsible for its cell-type specific expression. AAV vectors that contain various Avp gene promoter deletion constructs using EGFP as the reporter were stereotaxically injected into the rat SON. Two weeks following the injection immunohistochemical assays of EGFP expression from these constructs were done to determine whether the expressed EGFP reporter co-localizes with either the Oxt- or Avp-immunoreactivity in the MCNs. The results identify three major enhancer domains located at -2.0 to -1.5 kbp, -1.5 to -950 bp, and -950 to -543 bp in the Avp gene promoter that regulate the expression in Avp MCNs. The results also show that cell-type specific expression in Avp MCNs is maintained in constructs containing at least 288 bp of the promoter region upstream of the transcription start site, but this specificity is lost at 116 bp and below. Based on these data, we hypothesize that the -288 bp to -116 bp domain contains an Avp MCN specific activator and a possible repressor that inhibits expression in Oxt-MCNs, thereby leading to the cell-type specific expression of the Avp gene only in the Avp-MCNs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.