SynthesisPloS one2012
Utility of the trnH-psbA intergenic spacer region and its combinations as plant DNA barcodes: a meta-analysis.
Synthesis in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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74 citing papers in PubMed.
- Chloroplast Genome Sequence of Snap Bean (International journal of molecular sciences · 2026Article
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- Integrating Multivariate Analysis and DNA Barcoding for Amaranth Germplasm Characterization and Promising Genotype Selection.Plants (Basel, Switzerland) · 2026Article
- Article
- High-Resolution Chloroplast SNV Profiling of 409 Grapevine (International journal of molecular sciences · 2026Article
- Comparative analysis of monoterpene indole alkaloid composition and genotypic variation in ThaiFrontiers in plant science · 2026Article
- A universal DNA-barcode approach to aid species identification in the family Cyperaceae.Frontiers in plant science · 2026Article
- Article
- DNA Barcoding Provides Taxonomic Clues for Identifying Five EndangeredPlants (Basel, Switzerland) · 2025Article
- The Potential of Universal Primers for Barcoding of Subtropical Crops: Actinidia, Feijoa, Citrus, and Tea.International journal of molecular sciences · 2025Article
- The rps15-rpl32 intergenic locus is a phylogeographic marker for Latin American Zea mays landrace varieties and subspecies.BMC genomics · 2025Article
- DNA-Barcoding for Cultivar Identification and Intraspecific Diversity Analysis of Agricultural Crops.International journal of molecular sciences · 2025Review
- Applications and challenges of DNA barcoding in rapid radiation groups:Chinese herbal medicines · 2025Article
- Authentication of the Botanical Origin of Honey:Journal of agricultural and food chemistry · 2025Article
- Assessing the accuracy of cp-DNA barcodes in Colchicum species identification.Molecular biology reports · 2025Article
- Identification of Indigenous ThaiPlants (Basel, Switzerland) · 2025Article
- Chloroplast Genome Evolution of Hamamelidaceae at Subfamily Level.Ecology and evolution · 2025Article
- Insights from DNA Barcodes-Based Phylogenetic Analysis of Medicinal Plants and Estimation of Their Conservation Status: A Case Study in the Tianshan Wild Forest, China.Plants (Basel, Switzerland) · 2025Article
- A critical review of conventional and emerging technologies for the detection of contaminants, allergens and adulterants in plant-based milk alternatives.Current research in food science · 2025Review
- Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe trnH-psbA intergenic spacer region has been used in many DNA barcoding studies. However, a comprehensive evaluation with rigorous sequence preprocessing and statistical testing on the utility of trnH-psbA and its combinations as DNA barcodes is lacking. METHODOLOGY/PRINCIPAL
findingsSequences were searched from GenBank for a meta-analysis on the usefulness of trnH-psbA and its combinations as DNA barcodes. After preprocessing, we constructed full and matching data sets that contained 17 983 trnH-psbA sequences and 2190 sets of trnH-psbA, matK, rbcL, and ITS2 sequences from the same sample, repectively. These datasets were used to analyze the ability of trnH-psbA and its combinations to discriminate species by the BLAST and BLAST+P methods. The Fisher's exact test was used to evaluate the significance of performance differences. For the full data set, the identification success rates of trnH-psbA exceeded 70% in 18 families and 12 genera, respectively. For the matching data set, the identification rates of trnH-psbA were significantly higher than those of the other loci in two families and four genera. Similarly, the identification rates of trnH-psbA+ITS2 were significantly higher than those of matK+rbcL in 18 families and 21 genera. CONCLUSION/SIGNIFICANE: This study provides valuable information on the higher utility of trnH-psbA and its combinations. We found that trnH-psbA+ITS2 combination performs better or equally well compared with other combinations in most taxonomic groups investigated. This information will guide the optimal usage of trnH-psbA and its combinations for species identification.
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