ArticleThe Journal of reproduction and development2013
Ascorbic acid improves the developmental competence of porcine oocytes after parthenogenetic activation and somatic cell nuclear transplantation.
Article in The Journal of reproduction and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 91 citations in OpenAlex.
- Effects of ascorbic acid and cysteine supplementation on preimplantation embryo development and oxidative stress-related gene expression in dromedary camels.BMC veterinary research · 2025Article
- Vitamin C Improves Oocyte In Vitro Maturation and Potentially Changes Embryo Quality in Cattle.Veterinary sciences · 2024Article
- Improving the meiotic competence of small antral follicle-derived porcine oocytes by using dibutyryl-cAMP and melatonin.Animal bioscience · 2024Article
- Article
- Effects of Nesting Material Provision and High-Dose Vitamin C Supplementation during the Peripartum Period on Prepartum Nest-Building Behavior, Farrowing Process, Oxidative Stress Status, Cortisol Levels, and Preovulatory Follicle Development in Hyperprolific Sows.Antioxidants (Basel, Switzerland) · 2024Article
- Assessment of developmental rate of mouse embryos yielded from in vitro fertilization of the oocyte with treatment of melatonin and vitamin C simultaneously.BMC women's health · 2023Article
- Beneficial Effects of Catalpol Supplementation during In Vitro Maturation of Porcine Cumulus-Oocyte Complexes.Antioxidants (Basel, Switzerland) · 2023Article
- - Invited Review - Reproductive technologies needed for the generation of precise gene-edited pigs in the pathways from laboratory to farm.Animal bioscience · 2023Article
- Article
- Lycopene Supplementation to Serum-Free Maturation Medium Improves In Vitro Bovine Embryo Development and Quality and Modulates Embryonic Transcriptomic Profile.Antioxidants (Basel, Switzerland) · 2022Article
- Impact of L-carnitine supplementation on theVeterinary world · 2021Article
- Effect of oxygen tension and antioxidants on the developmental competence of buffalo oocytes culturedVeterinary world · 2021Article
- Antioxidant Nobiletin Enhances Oocyte Maturation and Subsequent Embryo Development and Quality.International journal of molecular sciences · 2020Article
- Ultrastructural Characterization of Porcine Growing and In Vitro Matured Oocytes.Animals : an open access journal from MDPI · 2020Article
- Vitamin C Protects Porcine Oocytes From Microcystin-LR Toxicity During Maturation.Frontiers in cell and developmental biology · 2020Article
- Obesity leads to distinct metabolomic signatures in follicular fluid of women undergoing in vitro fertilization.American journal of physiology. Endocrinology and metabolism · 2019Article
- Ascorbic acid improves parthenogenetic embryo development through TET proteins in mice.Bioscience reports · 2019Article
- Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes.Scientific reports · 2018Article
- C-Phycocyanin protects against mitochondrial dysfunction and oxidative stress in parthenogenetic porcine embryos.Scientific reports · 2017Article
- Vitamin C in Stem Cell Biology: Impact on Extracellular Matrix Homeostasis and Epigenetics.Stem cells international · 2017Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, a dose-response assessment was performed to understand the relation between supplementation of media with L-ascorbic acid or vitamin C and porcine oocyte maturation and the in vitro development of parthenotes (PA) and handmade cloned (HMC) embryos. Various concentrations (0, 25, 50 and 100 μg/ml) of vitamin C supplemented in in vitro maturation (IVM) and culture (IVC) media were tested. None of these vitamin C additions affected nuclear maturation of oocytes, yet supplementation at 50 μg/ml led to significantly increased intracellular glutathione (GSH) levels and reduced reactive oxygen species (ROS). When cultured in IVM- and/or IVC-supplemented media, the group supplemented with 50 μg/ml of vitamin C showed improved cleavage rates, blastocyst rates and total cell numbers per blastocyst (P<0.05) compared with other groups (control, 25 μg/ml and 100 μg/ml). In contrast, supplementation with 50 μg/ml vitamin C decreased (P<0.05) the apoptosis index as compared with the groups supplemented with 100 μg/ml. In addition, even with a lower blastocyst rate to start with (37.6 vs. 50.3%, P<0.05), supplementation of HMC embryos with vitamin C ameliorated their blastocyst quality to the extent of PA embryos as indicated by their total cell numbers (61.2 vs. 59.1). Taken together, an optimized concentration of vitamin C supplementation in the medium not only improves blastocyst rates and total cell numbers but also reduces apoptotic indices, whereas overdosages compromise various aspects of the development of parthenotes and cloned porcine embryos.
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Registered trials
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