ArticleBMC research notes2012
In silico vs in vitro analysis of primer specificity for the detection of Gardnerella vaginalis, Atopobium vaginae and Lactobacillus spp.
Article in BMC research notes, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Detection of coronaviruses in insectivorous bats of Fore-Caucasus, 2021.Scientific reports · 2023Pooled it
- Metagenomic next-generation sequencing contributes to the diagnosis ofBiomedical reports · 2026Article
- Editorial: Vaginal dysbiosis and biofilms, volume II.Frontiers in cellular and infection microbiology · 2025Article
- Nanopore-based amplicon sequencing for rapid detection and identification ofFrontiers in microbiology · 2025Article
- Ecology- and genome-based identification of theApplied and environmental microbiology · 2024Article
- Genomic and ecological approaches to identify theFrontiers in microbiology · 2024Article
- A clash of quorum sensing vs quorum sensing inhibitors: an overview and risk of resistance.Archives of microbiology · 2023Review
- Evaluation of Modulatory Activities of Lactobacillus crispatus Strains in the Context of the Vaginal Microbiota.Microbiology spectrum · 2022Article
- Clustering Analysis of the Multi-Microbial Consortium byFrontiers in cellular and infection microbiology · 2022Article
- Vaginal microbiota evaluation and prevalence of key pathogens in ecuadorian women: an epidemiologic analysis.Scientific reports · 2020Article
- Schistosoma species detection by environmental DNA assays in African freshwaters.PLoS neglected tropical diseases · 2020Article
- Development of a fixation-free fluorescenceBiology methods & protocols · 2020Article
- Article
- Vaginal Microbiota Evaluation and Lactobacilli Quantification by qPCR in Pregnant and Non-pregnant Women: A Pilot Study.Frontiers in cellular and infection microbiology · 2020Article
- An update on the role of Atopobium vaginae in bacterial vaginosis: what to consider when choosing a treatment? A mini review.Archives of gynecology and obstetrics · 2019Review
- Specific and Sensitive Primers Developed by Comparative Genomics to Detect Bacterial Pathogens in Grains.The plant pathology journal · 2018Article
- Article
- Prevalence of bacterial vaginosis in Portuguese pregnant women and vaginal colonization byPeerJ · 2017Article
- Using an in-vitro biofilm model to assess the virulence potential of bacterial vaginosis or non-bacterial vaginosis Gardnerella vaginalis isolates.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacterial vaginosis (BV) is a common pathology of women in reproductive age that can lead to serious health complications, and is associated with shifts in the normal microflora from predominance of Lactobacillus spp. to a proliferation of other anaerobes such as G. vaginalis and A vaginae, which can be detected by PCR. The optimal PCR pathogen detection assay relies mainly on the specificity and sensitivity of the primers used.
findingsHere we demonstrate that in silico analytical testing of primer specificity is not a synonym to in vitro analytical specificity by testing a range of published and newly designed primers with both techniques for the detection of BV-associated microorganisms.
conclusionsBy testing primer in vitro specificity with a sufficient range of bacterial strains, we were able to design primers with higher specificity and sensitivity. Also by comparing the results obtained for the newly designed primers with other previously published primers, we confirmed that in silico analysis is not sufficient to predict in vitro specificity. As such care must be taken when choosing the primers for a detection assay.
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Registered trials
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