ArticlePloS one2012
Egr2::cre mediated conditional ablation of dicer disrupts histogenesis of mammalian central auditory nuclei.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Dicer Deletion in the Ear Can Cut Most Neurons and Their Innervation of Hair Cells to Project to the Ear and the Brainstem.International journal of molecular sciences · 2026Article
- Cellular and synaptic specializations for sub-millisecond precision in the mammalian auditory brainstem.Frontiers in cellular neuroscience · 2025Review
- Shared and organ-specific gene-expression programs during the development of the cochlea and the superior olivary complex.RNA biology · 2023Article
- Loss of miR-183/96 Alters Synaptic Strength via Presynaptic and Postsynaptic Mechanisms at a Central Synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Talking back: Development of the olivocochlear efferent system.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Volumes of cochlear nucleus regions in rodents.Hearing research · 2016Article
- Functional Role of γ-Crystallin N in the Auditory Hindbrain.PloS one · 2016Article
- L-type Calcium Channel Cav1.2 Is Required for Maintenance of Auditory Brainstem Nuclei.The Journal of biological chemistry · 2015Article
- The gene regulatory networks underlying formation of the auditory hindbrain.Cellular and molecular life sciences : CMLS · 2015Review
- Evolving gene regulatory networks into cellular networks guiding adaptive behavior: an outline how single cells could have evolved into a centralized neurosensory system.Cell and tissue research · 2015Review
- Dicer expression is essential for adult midbrain dopaminergic neuron maintenance and survival.Molecular and cellular neurosciences · 2014Article
- MicroRNAs in sensorineural diseases of the ear.Frontiers in molecular neuroscience · 2013Review
- Time-dependent gene expression analysis of the developing superior olivary complex.The Journal of biological chemistry · 2013Article
- L-type CaV1.2 deletion in the cochlea but not in the brainstem reduces noise vulnerability: implication for CaV1.2-mediated control of cochlear BDNF expression.Frontiers in molecular neuroscience · 2013Article
- Approaches to manipulating microRNAs in neurogenesis.Frontiers in neuroscience · 2012Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histogenesis of the auditory system requires extensive molecular orchestration. Recently, Dicer1, an essential gene for generation of microRNAs, and miR-96 were shown to be important for development of the peripheral auditory system. Here, we investigated their role for the formation of the auditory brainstem. Egr2::Cre-mediated early embryonic ablation of Dicer1 caused severe disruption of auditory brainstem structures. In adult animals, the volume of the cochlear nucleus complex (CNC) was reduced by 73.5%. This decrease is in part attributed to the lack of the microneuronal shell. In contrast, fusiform cells, which similar to the granular cells of the microneural shell are derived from Egr2 positive cells, were still present. The volume reduction of the CNC was already present at birth (67.2% decrease). The superior olivary complex was also drastically affected in these mice. Nissl staining as well as Vglut1 and Calbindin 1 immunolabeling revealed that principal SOC nuclei such as the medial nucleus of the trapezoid body and the lateral superior olive were absent. Only choline acetyltransferase positive neurons of the olivocochlear bundle were observed as a densely packed cell group in the ventrolateral area of the SOC. Mid-embryonic ablation of Dicer1 in the ventral cochlear nucleus by Atoh7::Cre-mediated recombination resulted in normal formation of the cochlear nucleus complex, indicating an early embryonic requirement of Dicer1. Quantitative RT-PCR analysis of miR-96 demonstrated low expression in the embryonic brainstem and up-regulation thereafter, suggesting that other microRNAs are required for proper histogenesis of the auditory brainstem. Together our data identify a critical role of Dicer activity during embryonic development of the auditory brainstem.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.