ArticleJournal of clinical microbiology2013
Detection of Campylobacter species and Arcobacter butzleri in stool samples by use of real-time multiplex PCR.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Application of a microbial and pathogen source tracking toolbox to identify infrastructure problems in stormwater drainage networks: a case study.Microbiology spectrum · 2024Article
- Occurrence, Sources and Virulence Potential ofEnvironmental science & technology · 2024Article
- Comparison of the Diagnostic Accuracy of Three Real-Time PCR Assays for the Detection ofMicroorganisms · 2023Article
- A Case of Persistent Diarrhea in a Man with the Molecular Detection of VariousPathogens (Basel, Switzerland) · 2020Article
- Real-time quantitative PCR assay development and application for assessment of agricultural surface water and various fecal matter for prevalence of Aliarcobacter faecis and Aliarcobacter lanthieri.BMC microbiology · 2020Article
- Outcomes and clinical relevance of stool multiplex bacterial polymerase chain reaction in patients with acute diarrhea: single center experience.The Korean journal of internal medicine · 2020Article
- Article
- Impact of the BioFire FilmArray gastrointestinal panel on patient care and infection control.PloS one · 2020Article
- Campylobacter culture fails to correctly detect Campylobacter in 30% of positive patient stool specimens compared to non-cultural methods.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2019Article
- The Use of a DNA-Intercalating Dye for Quantitative Detection of ViableFrontiers in microbiology · 2019Article
- Exploiting Bacterial Whole-Genome Sequencing Data for Evaluation of Diagnostic Assays: Campylobacter Species Identification as a Case Study.Journal of clinical microbiology · 2016Article
- Enteropathogen infections in canine puppies: (Co-)occurrence, clinical relevance and risk factors.Veterinary microbiology · 2016Article
- Evaluation of Various Campylobacter-Specific Quantitative PCR (qPCR) Assays for Detection and Enumeration of Campylobacteraceae in Irrigation Water and Wastewater via a Miniaturized Most-Probable-Number-qPCR Assay.Applied and environmental microbiology · 2016Article
- Comparative Detection and Quantification of Arcobacter butzleri in Stools from Diarrheic and Nondiarrheic People in Southwestern Alberta, Canada.Journal of clinical microbiology · 2016Article
- An Improved Culture Method for Selective Isolation of Campylobacter jejuni from Wastewater.Frontiers in microbiology · 2016Article
- Global Epidemiology of Campylobacter Infection.Clinical microbiology reviews · 2015Review
- Development of a comparative genomic fingerprinting assay for rapid and high resolution genotyping of Arcobacter butzleri.BMC microbiology · 2015Article
- Update on the burden of Campylobacter in developing countries.Current opinion in infectious diseases · 2014Review
- Detection of Campylobacter in stool and determination of significance by culture, enzyme immunoassay, and PCR in developing countries.Journal of clinical microbiology · 2014Article
- A severe case of persistent diarrhoea associated with Arcobacter cryaerophilus but attributed to Campylobacter sp. and a review of the clinical incidence of Arcobacter spp.New microbes and new infections · 2014Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presence of Campylobacter (or Campylobacter-like) species in stools from patients suspected of infectious gastroenteritis (n = 493) was investigated using real-time PCR for detection of Arcobacter butzleri (hsp60 gene), Campylobacter coli (ceuE gene), Campylobacter jejuni (mapA), five acknowledged pathogenic Campylobacter spp. (C16S_Lund assay), and the Campylobacter genus (C16S_LvI assay). In total, 71.4% of the samples were positive for Campylobacter DNA (n = 352) by a Campylobacter genus-specific (C16S_LvI) assay. A total of 23 samples (4.7%) were positive in the C16S_Lund assay, used for detection of C. jejuni, C. coli, C. lari, C. upsaliensis, and C. hyointestinalis. Subsequent identification of these samples yielded detection frequencies (DF) of 4.1% (C. jejuni), 0.4% (C. coli), and 0.4% (C. upsaliensis). The DF of A. butzleri was 0.4%. Interestingly, sequencing of a subgroup (n = 46) of C16S_LvI PCR-positive samples resulted in a considerable number of Campylobacter concisus-positive samples (n = 20). PCR-positive findings with the C16S_Lund and C. jejuni/C. coli-specific assays were associated with more serious clinical symptoms (diarrhea and blood). Threshold cycle (C(T)) values of C. jejuni/C. coli PCR-positive samples were comparable to those of the C16S_Lund PCR (P = 0.21). C(T) values for both assays were significantly lower than those of the C16S_LvI assay (P < 0.001 and P < 0.00001, respectively). In conclusion, this study demonstrated that in combination, the C. jejuni/C coli-specific assays and the C16S_Lund assay are both useful for routine screening purposes. Furthermore, the DF of the emerging pathogen C. concisus was at least similar to the DF of C. jejuni.
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