ArticleJournal of virology2013
Systematic identification of H274Y compensatory mutations in influenza A virus neuraminidase by high-throughput screening.
Article in Journal of virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed.
- Concepts of RNA virus evolution for the design of better antiviral countermeasures.Nature reviews. Microbiology · 2026Review
- Scanning the horizon: deep mutational scanning approaches in virology.Journal of virology · 2026Review
- In-silico assessment of high-risk non-synonymous SNPs in ADAMTS3 gene associated with Hennekam syndrome and their impact on protein stability and function.BMC bioinformatics · 2023Article
- Predicting Permissive Mutations That Improve the Fitness of A(H1N1)pdm09 Viruses Bearing the H275Y Neuraminidase Substitution.Journal of virology · 2022Article
- Next-generation T cell-activating vaccination increases influenza virus mutation prevalence.Science advances · 2022Article
- Identification of a Permissive Secondary Mutation That Restores the Enzymatic Activity of Oseltamivir Resistance Mutation H275Y.Journal of virology · 2022Article
- Antigenic evolution of human influenza H3N2 neuraminidase is constrained by charge balancing.eLife · 2021Article
- Saturation Mutagenesis of the Transmembrane Region of HokC inInternational journal of molecular sciences · 2021Article
- Applications of Deep Mutational Scanning in Virology.Viruses · 2021Review
- Concerns about SARS-CoV-2 evolution should not hold back efforts to expand vaccination.Nature reviews. Immunology · 2021Review
- Quantifying the Evolutionary Constraints and Potential of Hepatitis C Virus NS5A Protein.mSystems · 2021Article
- MaveDB: an open-source platform to distribute and interpret data from multiplexed assays of variant effect.Genome biology · 2019Article
- Multiplexed assays of variant effects contribute to a growing genotype-phenotype atlas.Human genetics · 2018Review
- Rapid and simple detection of Tamiflu-resistant influenza virus: Development of oseltamivir derivative-based lateral flow biosensor for point-of-care (POC) diagnostics.Scientific reports · 2018Article
- Intracellular interleukin-32γ mediates antiviral activity of cytokines against hepatitis B virus.Nature communications · 2018Article
- Mutation and Epistasis in Influenza Virus Evolution.Viruses · 2018Review
- Circular permutation profiling by deep sequencing libraries created using transposon mutagenesis.Nucleic acids research · 2018Article
- Characterization of influenza virus variants induced by treatment with the endonuclease inhibitor baloxavir marboxil.Scientific reports · 2018Article
- Mapping the Evolutionary Potential of RNA Viruses.Cell host & microbe · 2018Review
- Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Compensatory mutations contribute to the appearance of the oseltamivir resistance substitution H274Y in the neuraminidase (NA) gene of H1N1 influenza viruses. Here, we describe a high-throughput screening method utilizing error-prone PCR and next-generation sequencing to comprehensively screen NA genes for H274Y compensatory mutations. We found four mutations that can either fully (R194G, E214D) or partially (L250P, F239Y) compensate for the fitness deficiency of the H274Y mutant. The compensatory effect of E214D is applicable in both seasonal influenza virus strain A/New Caledonia/20/1999 and 2009 pandemic swine influenza virus strain A/California/04/2009. The technique described here has the potential to profile a gene at the single-nucleotide level to comprehend the dynamics of mutation space and fitness and thus offers prediction power for emerging mutant species.
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Registered trials
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