ArticleJournal of assisted reproduction and genetics2012
In vitro culture and morphological characterization of prepubertal buffalo (Bubalus bubalis) putative spermatogonial stem cell.
Article in Journal of assisted reproduction and genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 39 citations in OpenAlex.
- Recent Progress of In Vitro 3D Culture of Male Germ Stem Cells.Journal of functional biomaterials · 2023Review
- Effects of Growth Factors on In Vitro Culture of Neonatal Piglet Testicular Tissue Fragments.Cells · 2023Article
- Genomic Identification, Evolution, and Expression Analysis of Bromodomain Genes Family in Buffalo.Genes · 2022Article
- Current scenario and challenges ahead in application of spermatogonial stem cell technology in livestock.Journal of assisted reproduction and genetics · 2021Review
- The role of Rho-associated kinase inhibitor, Y-27632 on primary culture of ovine spermatogonial stem cells.Animal reproduction · 2021Article
- EGF, GDNF, and IGF-1 influence the proliferation and stemness of ovine spermatogonial stem cells in vitro.Journal of assisted reproduction and genetics · 2020Article
- Effects of different culture systems on the culture of prepuberal buffalo (Journal of veterinary science · 2020Article
- Bovid microRNAs involved in the process of spermatogonia differentiation into spermatocytes.International journal of biological sciences · 2020Article
- In vitro culture and characterization of spermatogonial stem cells on Sertoli cell feeder layer in goat (Capra hircus).Journal of assisted reproduction and genetics · 2014Article
- Germline stem cells: toward the regeneration of spermatogenesis.Fertility and sterility · 2014Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeSpermatogonial stem cells (SSCs) have the unique ability both to self-renew and to produce progeny that undergo differentiation to spermatozoa. The present study has been carried out to develop a method to purify and enrich the pure populations of spermatogonial stem cell like cells in buffalo.
methodsThe spermatogonial cells were isolated from testes of 3-7 month old buffalo calves and disaggregated by double enzymatic digestion. Mixed population of isolated cells were then plated on Datura stramonium agglutinin (DSA) lectin coated dishes for attachment of Sertoli cells. The desired cells were obtained from suspension medium after 18 h of incubation and then loaded on discontinuous density gradient using percoll (20-65 %) and different types of spermatogonia cells were obtained at interface of each layer. These cells were cultured in vitro.
resultsSpermatogonial cells isolated have spherical outline and two or three eccentrically placed nucleoli, created a colony after proliferation during first week or immediately after passage. After 7-10 days of culture, the resulted developed colonies of spermatogonial cells expressed the spermatogonial specific genes like Plzf and VASA; and other pluripotency related markers viz. alkaline phosphtase, DBA, CD9, CD90, SSEA-1, OCT-4, NANOG and REX-1.
conclusionOur results show that the isolated putative spermatogonial stem cells exhibit the expression of pluripotency related and spermatogonial specific genes. This study may help to establish a long term culture system for buffalo spermatogonia.
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