ArticleCurrent protocols in protein science2012
Preparation and extraction of insoluble (inclusion-body) proteins from Escherichia coli.
Article in Current protocols in protein science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
53 citing papers in PubMed.
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- Predatory myxobacteria lay at the cross-roads of metallo-β-lactamase evolution.Communications biology · 2026Article
- Lysis and IB washing procedures significantly impact the recovery of rHumanized ranibizumab from inclusion bodies expressed in Escherichia coli.Bioprocess and biosystems engineering · 2026Article
- Article
- The Expression, Purification, Spectroscopic Characterization, and Membrane Topology Classification of KCNE4 from RecombinantThe journal of physical chemistry. B · 2025Article
- Recombinant expression, downstream optimization, and therapeutic evaluation of recombinant human interleukin-37 for cancer therapy.Biotechnology letters · 2024Article
- HylS', a fragment of truncated hyaluronidase ofVirulence · 2024Article
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- FAXC interacts with ANXA2 and SRC in mitochondria and promotes tumorigenesis in cholangiocarcinoma.Cancer science · 2024Article
- A distinct, high-affinity, alkaline phosphatase facilitates occupation of P-depleted environments by marine picocyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Essential factors, advanced strategies, challenges, and approaches involved for efficient expression of recombinant proteins in Escherichia coli.Archives of microbiology · 2024Review
- Simultaneous Purification of Human Interferon Alpha-2b and Serum Albumin Using Bioprivileged Fluorinated Ionic Liquid-Based Aqueous Biphasic Systems.International journal of molecular sciences · 2024Article
- Functional Comparison of Three Chitinases from Symbiotic Bacteria of Entomopathogenic Nematodes.Toxins · 2024Article
- Efficient strategy to isolate exosomes using anti-CD63 antibodies conjugated to gold nanoparticles.AMB Express · 2023Article
- Detection of Human Paragonimiasis by ELISA Using Recombinant Paragonimus westermani Cysteine Protease 7.The American journal of tropical medicine and hygiene · 2023Article
- Fundamental insights in early-stage inclusion body formation.Microbial biotechnology · 2023Article
- Factors involved in heterologous expression of proteins in E. coli host.Archives of microbiology · 2023Review
- Molecular Cloning, Expression, and Functional Analysis of Glycosyltransferase (TbUGGT) Gene fromMolecules (Basel, Switzerland) · 2022Article
- CRISPR-Cas9 Mediated Knockout ofMicroorganisms · 2022Article
- Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
High-level expression of many recombinant proteins in Escherichia coli leads to the formation of highly aggregated protein commonly referred to as inclusion bodies. Inclusion bodies are normally formed in the cytoplasm; however, if a secretion vector is used, they can form in the periplasmic space. Inclusion bodies can be recovered from cell lysates by low-speed centrifugation. Following pre-extaction (or washing), protein is extracted from washed pellets using guanidine⋅HCl. The solubilized and unfolded protein is either directly folded or further purified by gel filtration in the presence of guanidine⋅HCl as described in this unit. A support protocol describes the removal of guanidine⋅HCl from column fractions so they can be monitored by SDS-PAGE.
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Registered trials
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