ArticleAnalytical and bioanalytical chemistry2013
The effect of optical substrates on micro-FTIR analysis of single mammalian cells.
Article in Analytical and bioanalytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Engineering Nanomaterials for Raman Bioimaging and Theranostics: Advances, Challenges, and Future Directions.ACS omega · 2025Review
- Photochemistry of Receptor-Bound Flavin Resolved in Living Human Cells by Infrared Spectroscopy.Journal of the American Chemical Society · 2025Article
- Benchmarking classification abilities of novel optical photothermal IR spectroscopy at the single-cell level with bulk FTIR measurements.Analytical methods : advancing methods and applications · 2024Article
- Evaluation of the Cytotoxic Effect of PdInternational journal of molecular sciences · 2023Article
- Synchrotron Radiation FTIR Microspectroscopy Study of Biomolecular Alterations in Vincristine-Treated WRL68 Cells at the Single-Cell Level.ACS omega · 2022Article
- Oxidative Stress- and Autophagy-Inducing Effects of PSI-LHCI fromBiotech (Basel (Switzerland)) · 2022Article
- Who's Who? Discrimination of Human Breast Cancer Cell Lines by Raman and FTIR Microspectroscopy.Cancers · 2022Article
- An infrared spectral biomarker accurately predicts neurodegenerative disease class in the absence of overt symptoms.Scientific reports · 2021Article
- Raman spectral cytopathology for cancer diagnostic applications.Nature protocols · 2021Article
- Graphene Oxide-Induced Protein Conformational Change in Nasopharyngeal Carcinoma Cells: A Joint Research on Cytotoxicity and Photon Therapy.Materials (Basel, Switzerland) · 2021Article
- Development of an inexpensive Raman-compatible substrate for the construction of a microarray screening platform.The Analyst · 2020Article
- The Bouguer-Beer-Lambert Law: Shining Light on the Obscure.Chemphyschem : a European journal of chemical physics and physical chemistry · 2020Review
- Subcellular mapping of living cells via synchrotron microFTIR and ZnS hemispheres.Analytical and bioanalytical chemistry · 2018Article
- Biochemical profiling of rat embryonic stem cells grown on electrospun polyester fibers using synchrotron infrared microspectroscopy.Analytical and bioanalytical chemistry · 2018Article
- Detection of mycoplasma in contaminated mammalian cell culture using FTIR microspectroscopy.Analytical and bioanalytical chemistry · 2018Article
- Label-free monitoring of cell death induced by oxidative stress in living human cells using terahertz ATR spectroscopy.Biomedical optics express · 2018Article
- Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light.Journal of visualized experiments : JoVE · 2017Article
- Using Raman spectroscopy to characterize biological materials.Nature protocols · 2016Article
- Microfluidic approaches to synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectral microscopy of living biosystems.Protein and peptide letters · 2016Article
- Cold shock induces apoptosis of dorsal root ganglion neurons plated on infrared windows.The Analyst · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of individual cells with infrared (IR) microspectroscopy often requires living cells to be cultured directly onto a suitable substrate. The surface effect of the specific substrates on the cell growth-viability and associated biochemistry-as well as on the IR analysis-spectral interference and optical artifacts-is all too often ignored. Using the IR beamline, MIRIAM (Diamond Light Source, UK), we show the importance of the substrate used for IR absorption spectroscopy by analyzing two different cell lines cultured on a range of seven optical substrates in both transmission and reflection modes. First, cell viability measurements are made to determine the preferable substrates for normal cell growth. Successively, synchrotron radiation IR microspectroscopy is performed on the two cell lines to determine any genuine biochemically induced changes or optical effect in the spectra due to the different substrates. Multivariate analysis of spectral data is applied on each cell line to visualize the spectral changes. The results confirm the advantage of transmission measurements over reflection due to the absence of a strong optical standing wave artifact which amplifies the absorbance spectrum in the high wavenumber regions with respect to low wavenumbers in the mid-IR range. The transmission spectra reveal interference from a more subtle but significant optical artifact related to the reflection losses of the different substrate materials. This means that, for comparative studies of cell biochemistry by IR microspectroscopy, it is crucial that all samples are measured on the same substrate type.
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