ArticleEpigenetics2012
Interindividual variability and co-regulation of DNA methylation differ among blood cell populations.
Article in Epigenetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Epigenetics and human obesity.International journal of obesity (2005) · 2015Pooled it
- Epigenetic mechanisms and steroid-resistant nephrotic syndrome: The future potential for SRNS diagnosis.iScience · 2026Review
- Reply to: Validation of blood-based detection of breast cancer highlights importance for cross-population validation.Nature communications · 2025Article
- Systematic evaluation of methylation-based cell type deconvolution methods for plasma cell-free DNA.Genome biology · 2024Article
- Epigenome-wide association studies of occupational exposure to benzene and formaldehyde.Epigenetics · 2022Article
- Epigenetic regulation of pediatric and neonatal immune responses.Pediatric research · 2022Review
- Latency-associated DNA methylation patterns among HIV-1 infected individuals with distinct disease progression courses or antiretroviral virologic response.Scientific reports · 2021Article
- Review
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- Epigenetic underpinnings of developmental immunotoxicity and autoimmune disease.Current opinion in toxicology · 2018Article
- Conserved DNA Methylation Signatures in Early Maternal Separation and in Twins Discordant for COScientific reports · 2018Article
- Factors Affecting the Immunity to Respiratory Syncytial Virus: From Epigenetics to Microbiome.Frontiers in immunology · 2018Review
- Trichloroethylene-induced alterations in DNA methylation were enriched in polycomb protein binding sites in effector/memory CD4Environmental epigenetics · 2017Article
- Chronic exposure to trichloroethylene increases DNA methylation of the Ifng promoter in CD4Toxicology letters · 2016Article
- Cell type specific DNA methylation in cord blood: A 450K-reference data set and cell count-based validation of estimated cell type composition.Epigenetics · 2016Article
- DNA methylations of MC4R and HNF4α are associated with increased triglyceride levels in cord blood of preterm infants.Medicine · 2016Article
- Chronic exposure to water pollutant trichloroethylene increased epigenetic drift in CD4(+) T cells.Epigenomics · 2016Article
- Modulation of human allogeneic and syngeneic pluripotent stem cells and immunological implications for transplantation.Transplantation reviews (Orlando, Fla.) · 2016Review
- System-Wide Associations between DNA-Methylation, Gene Expression, and Humoral Immune Response to Influenza Vaccination.PloS one · 2016Article
- Trans-ancestry genome-wide association study identifies 12 genetic loci influencing blood pressure and implicates a role for DNA methylation.Nature genetics · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation regulates gene expression in a cell-type specific way. Although peripheral blood mononuclear cells (PBMCs) comprise a heterogeneous cell population, most studies of DNA methylation in blood are performed on total mononuclear cells. In this study, we investigated high resolution methylation profiles of 58 CpG sites dispersed over eight immune response genes in multiple purified blood cells from healthy adults and newborns. Adjacent CpG sites showed methylation levels that were increasingly correlated in adult blood vs. cord blood. Thus, while interindividual variability increases from newborn to adult blood, the underlying methylation changes may not be merely stochastic, but seem to be orchestrated as clusters of adjacent CpG sites. Multiple linear regression analysis showed that interindividual methylation variability was influenced by distance of average methylation levels to the closest border (0 or 100%), presence of transcription factor binding sites, CpG conservation across species and age. Furthermore, CD4+ and CD14+ cell types were negative predictors of methylation variability. Concerns that PBMC methylation differences may be confounded by variations in blood cell composition were justified for CpG sites with large methylation differences across cell types, such as in the IFN-γ gene promoter. Taken together, our data suggest that unsorted mononuclear cells are reasonable surrogates of CD8+ and, to a lesser extent, CD4+ T cell methylation in adult peripheral, but not in neonatal, cord blood.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.