ArticleMetabolism: clinical and experimental2013
The effect of varying ratios of docosahexaenoic acid and arachidonic acid in the prevention and reversal of biochemical essential fatty acid deficiency in a murine model.
Article in Metabolism: clinical and experimental, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- In defense of the Holman index: Defining fatty acid deficiency.Nutrition (Burbank, Los Angeles County, Calif.) · 2026Review
- Effects of dietary omega-3 fatty acids on bones of healthy mice.Clinical nutrition (Edinburgh, Scotland) · 2019Article
- Intravenous Fish Oil and Serum Fatty Acid Profiles in Pediatric Patients With Intestinal Failure-Associated Liver Disease.JPEN. Journal of parenteral and enteral nutrition · 2019Article
- Redefining essential fatty acids in the era of novel intravenous lipid emulsions.Clinical nutrition (Edinburgh, Scotland) · 2018Review
- Intravenous Fat Emulsion Formulations for the Adult and Pediatric Patient: Understanding the Differences.Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition · 2016Review
- Phenotypic divergence in two lines of L-Fabp-/- mice reflects substrain differences and environmental modifiers.American journal of physiology. Gastrointestinal and liver physiology · 2015Article
- Intravenous Lipid Emulsions in Parenteral Nutrition.Advances in nutrition (Bethesda, Md.) · 2015Review
- Potential role of dietary lipids in the prophylaxis of some clinical conditions.Archives of medical science : AMS · 2015Article
- Targeted lipidomic strategies for oxygenated metabolites of polyunsaturated fatty acids.Biochimica et biophysica acta · 2015Review
- Ω-3 fatty acids prevent hepatic steatosis, independent of PPAR-α activity, in a murine model of parenteral nutrition-associated liver disease.JPEN. Journal of parenteral and enteral nutrition · 2014Article
- Is there an optimal dose for dietary linoleic acid? Lessons from essential fatty acid deficiency supplementation and adipocyte functions in rats.Journal of physiology and biochemistry · 2014Article
- The Pex1-G844D mouse: a model for mild human Zellweger spectrum disorder.Molecular genetics and metabolism · 2014Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveEssential fatty acids (EFA) are necessary for growth, development, and biological function, and must be acquired through the diet. While linoleic acid (LA) and alpha-linolenic acid (ALA) have been considered the true EFAs, we previously demonstrated that docosahexaenoic acid (DHA) and arachidonic acid (AA) taken together as the sole source of dietary fatty acids can prevent biochemical essential fatty acid deficiency (EFAD). This study evaluates the effect of varying dietary ratios of DHA:AA in the prevention and reversal of biochemical EFAD in a murine model.
methodsUsing a murine model of EFAD, we provided mice with 2.1% of daily caloric intake in varying DHA:AA ratios (1:1, 5:1, 10:1, 20:1, 200:1, 100:0) for 19 days in association with a liquid high-carbohydrate fat-free diet to evaluate the effect on fatty acid profiles. In a second experiment, we evaluated the provision of varying DHA:AA ratios (20:1, 200:1, 100:0) on the reversal of biochemical EFAD.
resultsMice provided with DHA and AA had no evidence of biochemical EFAD, regardless of the ratio (1:1, 5:1, 10:1, 20:1, 200:1, 100:0) administered. Biochemical EFAD was reversed with DHA:AA ratios of 20:1, 200:1, and 100:0 following 3 and 5 weeks of dietary provision, although the 20:1 ratio was most effective in the reversal and stabilization of the triene:tetraene ratio.
conclusionProvision of DHA and AA, at 2.1% of daily caloric intake in varying ratios can prevent biochemical evidence of EFAD and hepatic steatosis over the short-term, with a ratio of 20:1 DHA:AA most effectively reversing EFAD.
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