ArticleThe Journal of clinical endocrinology and metabolism2013
Higher mitochondrial respiration and uncoupling with reduced electron transport chain content in vivo in muscle of sedentary versus active subjects.
Article in The Journal of clinical endocrinology and metabolism, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01911091 (Identification of Novel Skeletal Muscle-derived Factors That Promote Lipid Oxidation in Both Skeletal Muscle and Adipose Tissue), which is not on this map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Identification of Novel Skeletal Muscle-derived Factors That Promote Lipid Oxidation in Both Skeletal Muscle and Adipose Tissue
Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Molecular correlates of MRS-basedNMR in biomedicine · 2021Trial
- Effects of 12 Months of Caloric Restriction on Muscle Mitochondrial Function in Healthy Individuals.The Journal of clinical endocrinology and metabolism · 2017Trial
- Differences in Mitochondrial Coupling Reveal a Novel Signature of Mitohormesis in Muscle of Healthy Individuals.The Journal of clinical endocrinology and metabolism · 2016Trial
- Energy constraint on human health.Trends in endocrinology and metabolism: TEM · 2026Review
- Neither intracellular oxygen availability nor mitochondrial coupling explain lower oxidative capacity in older human vastus lateralis muscle in vivo.The Journal of physiology · 2026Article
- The brain-body energy conservation model of aging.Nature aging · 2024Review
- Effect of Sirt3 on hippocampal MnSOD activity, mitochondrial function, physiology, and cognition in an aged murine model.Behavioural brain research · 2023Article
- Physiology of Energy Expenditure in the Weight-Reduced State.Obesity (Silver Spring, Md.) · 2021Article
- Congestive Heart Failure Exhibited Higher BMI With Lower Energy Intake and Lower Physical Activity Level: Data From the National Health and Examination Nutrition Survey.Frontiers in cardiovascular medicine · 2021Article
- Impaired skeletal muscle mitochondrial bioenergetics and physical performance in chronic kidney disease.JCI insight · 2020Article
- Targeting the Mitochondria in Heart Failure: A Translational Perspective.JACC. Basic to translational science · 2020Review
- Metabolic adaptation is not observed after 8 weeks of overfeeding but energy expenditure variability is associated with weight recovery.The American journal of clinical nutrition · 2019Article
- Potential Causes of Elevated REE after High-Intensity Exercise.Medicine and science in sports and exercise · 2017Article
- Skeletal muscle bioenergetics in aging and heart failure.Heart failure reviews · 2017Review
- New Therapeutics to Modulate Mitochondrial Function in Neurodegenerative Disorders.Current pharmaceutical design · 2017Review
- CKD and Muscle Mitochondrial Energetics.American journal of kidney diseases : the official journal of the National Kidney Foundation · 2016Article
- Mitochondrial Coupling and Contractile Efficiency in Humans with High and Low V˙O2peaks.Medicine and science in sports and exercise · 2016Article
- Evaluation of in vivo mitochondrial bioenergetics in skeletal muscle using NMR and optical methods.Biochimica et biophysica acta · 2016Review
- Beetroot juice supplementation reduces the oxygen cost of exercise without improving mitochondrial efficiency: but how?The Journal of physiology · 2016Article
- Mitochondria to motion: optimizing oxidative phosphorylation to improve exercise performance.The Journal of experimental biology · 2016Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
Abstract
objectiveThis study investigated the disparity between muscle metabolic rate and mitochondrial metabolism in human muscle of sedentary vs. active individuals. RESEARCH DESIGN AND
methodsChronic activity level was characterized by a physical activity questionnaire and a triaxial accelerometer as well as a maximal oxygen uptake test. The ATP and O(2) fluxes and mitochondrial coupling (ATP/O(2) or P/O) in resting muscle as well as mitochondrial capacity (ATP(max)) were determined in vivo in human vastus lateralis muscle using magnetic resonance and optical spectroscopy on 24 sedentary and seven active subjects. Muscle biopsies were analyzed for electron transport chain content (using complex III as a representative marker) and mitochondrial proteins associated with antioxidant protection.
resultsSedentary muscle had lower electron transport chain complex content (65% of the active group) in proportion to the reduction in ATP(max) (0.69 ± 0.07 vs. 1.07 ± 0.06 mM sec(-1)) as compared with active subjects. This lower ATP(max) paired with an unchanged O(2) flux in resting muscle between groups resulted in a doubling of O(2) flux per ATP(max) (3.3 ± 0.3 vs. 1.7 ± 0.2 μM O(2) per mM ATP) that reflected mitochondrial uncoupling (P/O = 1.41 ± 0.1 vs. 2.1 ± 0.3) and greater UCP3/complex III (6.0 ± 0.7 vs. 3.8 ± 0.3) in sedentary vs. active subjects.
conclusionA smaller mitochondrial pool serving the same O(2) flux resulted in elevated mitochondrial respiration in sedentary muscle. In addition, uncoupling contributed to this higher mitochondrial respiration. This finding resolves the paradox of stable muscle metabolism but greater mitochondrial respiration in muscle of inactive vs. active subjects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.