ArticleProceedings of the National Academy of Sciences of the United States of America2012
Nucleosome-depleted chromatin gaps recruit assembly factors for the H3.3 histone variant.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
61 citing papers in PubMed, 91 citations in OpenAlex.
- IFN-α suppresses GATA3 transcription from a distal exon and promotes H3K27 trimethylation of the CNS-1 enhancer in human Th2 cells.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
- A genetic screen for regulators of Drosophila histone H1 binding and chromosome structure in vivo.Genetics · 2026Article
- ATRX: From Chromatin Remodeling to Disease.Genesis (New York, N.Y. : 2000) · 2025Review
- Sunflower seed-specific HSFA9 induces persistent chromatin accessibility during seed-to-seedling developmental transition.The Plant journal : for cell and molecular biology · 2025Article
- Article
- Remodeling of perturbed chromatin can initiate de novo transcriptional and post-transcriptional silencing.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- HIRA vs. DAXX: the two axes shaping the histone H3.3 landscape.Experimental & molecular medicine · 2024Review
- Multiple Roles of dXNP and dADD1-International journal of molecular sciences · 2023Review
- H3.3 contributes to chromatin accessibility and transcription factor binding at promoter-proximal regulatory elements in embryonic stem cells.Genome biology · 2023Article
- The nanoCUT&RUN technique visualizes telomeric chromatin in Drosophila.PLoS genetics · 2022Article
- The chromatin remodeller ATRX facilitates diverse nuclear processes, in a stochastic manner, in both heterochromatin and euchromatin.Nature communications · 2022Article
- The H3.3 chaperone Hira complex orchestrates oocyte developmental competence.Development (Cambridge, England) · 2022Article
- Cell Biology of Giant Cell Tumour of Bone: Crosstalk between m/wt Nucleosome H3.3, Telomeres and Osteoclastogenesis.Cancers · 2021Review
- The histone replacement gene His4r is involved in heat stress induced chromatin rearrangement.Scientific reports · 2021Article
- Cell Fate Decisions in the Wake of Histone H3 Deposition.Frontiers in cell and developmental biology · 2021Review
- An embryonic stem cell-specific heterochromatin state promotes core histone exchange in the absence of DNA accessibility.Nature communications · 2020Article
- Application of Recombination -Induced Tag Exchange (RITE) to study histone dynamics in human cells.Epigenetics · 2020Article
- Insights into HP1a-Chromatin Interactions.Cells · 2020Review
- H3.3 Nucleosome Assembly Mutants Display a Late-Onset Maternal Effect.Current biology : CB · 2020Article
- Chromatin Remodelers in the 3D Nuclear Compartment.Frontiers in genetics · 2020Review
1 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Most nucleosomes that package eukaryotic DNA are assembled during DNA replication, but chromatin structure is routinely disrupted in active regions of the genome. Replication-independent nucleosome replacement using the H3.3 histone variant efficiently repackages these regions, but how histones are recruited to these sites is unknown. Here, we use an inducible system that produces nucleosome-depleted chromatin at the Hsp70 genes in Drosophila to define steps in the mechanism of nucleosome replacement. We find that the Xnp chromatin remodeler and the Hira histone chaperone independently bind nucleosome-depleted chromatin. Surprisingly, these two factors are only displaced when new nucleosomes are assembled. H3.3 deposition assays reveal that Xnp and Hira are required for efficient nucleosome replacement, and double-mutants are lethal. We propose that Xnp and Hira recognize exposed DNA and serve as a binding platform for the efficient recruitment of H3.3 predeposition complexes to chromatin gaps. These results uncover the mechanisms by which eukaryotic cells actively prevent the exposure of DNA in the nucleus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.